FastQCFastQC Report
Thu 2 May 2024
EGAF00005475310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51872946
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTAATACCTGCCACCCCACTCTTAATCAGTGGTGGAAGAACGGTCTC692549713.350884293327006No Hit
CTCTTCAATCTCTTGCACTCAAAGCTTGTTAAGATAGTTAAGCGTGCATA27067145.217968534117958No Hit
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG13767462.6540732812823085No Hit
TGGATCAAGTTTAAATGACTGTGCTGCCCCTTTCACATCAAAGAACTACT13688802.6389093073680447No Hit
CTCATTAAAGCAGCCGGTGTAAATGTTGAGCCTTTTTGGCCTGGCTTGTT12953232.4971070661766537No Hit
TTGAAGACGTCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCTGCT11821532.2789393916435747No Hit
AGAAACAGACTGCCTGAATTGAGAATTTTGATTTTTTAAAGTGTGTTTCT10955872.1120585670996976No Hit
ATGCTGATGGCGAGGCTAACCTGGGCCTCCCACGAGAAGATGCACGAGGG10842332.090170471520935No Hit
AACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTGTGACTTC8956191.726562821398268No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCACCTGAA8264681.5932544104975261No Hit
TGGGATCTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACAT8037821.5495206306578386No Hit
ATCTCTGTGACTTCCTGGAGACTCACTTCCTAGATGAGGAAGTGAAGCTT7571561.4596356258616967No Hit
TTGTTGAGGTGGTCTGAATGTTCTGACATTAACAGTTTTCCATGAAAACG7120781.3727348356116114No Hit
AAGGCACAGGGAGAAGGGATAACCCTACACCCAGACCCCAGGCTGGACAT7056911.360422058928367No Hit
TAGAGACCGAGTGTCCTCAGTATATCAGGAAAAAGGGTGCAGACGTCTGG6239501.2028428075012358No Hit
ATGTCCACCATGACCCTCTTCCCACGCTGACCTGTGCTCCCTCCCCAATC6048051.1659353220462938No Hit
TCCGTCTTATTTCATTTCTGTAACAGTTGATATCTGGCTGTCCTTTTTAT5554781.0708433640919488No Hit
CTCTAGAGGCTGGCAAGAACCAGTTGTTTTGTCTTGCGGGTCTGTCAGGG5236881.0095590098160225No Hit
CAACAGAAAAACAATCTTATTCCGAGCATTCCAGTAACTTTTTTGTGTAT5040550.9717107642199461No Hit
GTTTCAGTGAACAAGTTTCAGCGGTTCAACTTTATAATAATTATAAATAA4947340.9537418599668505No Hit
GACTGTTCACAGACCTCAGTGATGTGAGCACTGTTGCTCAGGAGTGACAA4801870.9256983399400528No Hit
TAGGTGTCTGCCTTCACAAATGTCATTGTCTACTCCTAGAAGAACCAAAT4784460.9223420624693266No Hit
TACCCCCGTCCTCTTCGAGACCCCAGTCGTGATGTGGAGGAAGAGCCACC4075170.7856060459724035No Hit
GTGAGGAAGAAGGAGAGGAATACTAATTATCCATTCCTTTTGGCCCTGCA3872620.7465587167538161No Hit
CAAGCTCATTTCCTGGTATGACAACGAATTTGGCTACAGCAACAGGGTGG3757490.7243641030143151No Hit
GATGATGATGAAAGTCTTCTTGGACACTTAATGATTGTTGGCAAGAAATG3655660.7047334462168391No Hit
ACAATCCTTGACCTCAGTGAAAGCAGTCATCTTCAGCATTTTCCAGCCCT3638000.7013289740667515No Hit
TTTCCATGTAGAAAGGCAGGAATGTCTCCTAAGCTTTCCTGGACAGCAGA3530720.6806476732591975No Hit
ATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGAC3355180.6468072971988135No Hit
GGTAGGACTGTAGAAAAGGGAACTGAACATTCCAGAGCGTGTAGTGAATC3176520.6123654515399992No Hit
ACAATCCTTGACCTCAGTGAAAGCAGTCATCTTCAGCGTTTTCCAGCCCT3116780.6008488509598047No Hit
GCATATAGAGGCAAGAAAGAGATACCAAGATGCCATCTGTGTCCTGGTTA3043590.5867393766299682No Hit
CTGAATGTAACCTGCTAGCTCTCCGAAGGCCCTGCGGGCCTGGCTTGTGT2749280.5300026722985812No Hit
GACTGTCTAAAGGATGCCTGGATTCCTTGTTATCTCAGGACTCTAAATAC2442230.4708099671069385No Hit
CGCCATGATGTGTAACGAATTCTTTGAAGGCTTCCCAGATAAGCAGCCCA2358910.454747644369379No Hit
TGGTATTTTCAATAGCCACTAACCTTGCCTGGTACAGTATGGGGGTTGTA2345630.4521875430017026No Hit
GCAATAAGAAATGAACAAAAGATTGGAGCTGAAGACCTAAAGTGCTTGCT2227690.42945122106617967No Hit
GATCTCCTTCGCTGACTACAACCTGCTGGACTTGCTGCTGATCCATGAGG2154130.4152704186108882No Hit
CCTTCGATCTCCTGACGAGTTTATTGTTGGCCAAAACCAGGCTTTGATTG2087940.4025103953031702No Hit
GGCAGACCTGTTATCCTAAACTAGGGTTTTTAATGACCACAACAAGCAAG2058140.3967655895232941No Hit
AGACGGTTGAAACTAGAGATGGACAGGTTATCAACGAAACTTCTCAGCAT1990620.38374917052137353No Hit
TGACCCTTCAAGCTCTGTCCTTATCCATTACCTCAAAGCAGTCATTCCTT1794490.3459394806687864No Hit
TTCCCTCTCCTGTCCTTGTGTTGAAGGCAGTAAACTAAGGGTGTCAAGCC1694860.3267329370496906No Hit
CTACAAATGAGCTCTTGCAACAGAACTCACAGCCTTTTTACTTTTTTCCC1580140.30461736258434213No Hit
GACAGTGATTCCTGCAACTTGACCTTCAGGCTGGGAGAGGTGGAGAGCCA1522370.29348053607751523No Hit
GAGAACTGCTTCTAAGATGCCAGCATATACTGAAGTCTTTTCTGTCACCA1504770.2900876306504743No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCATCTGAA1456920.2808631690207069No Hit
GACAAAACGACACCAGCCAAACCAGCAGCCCCTCAGCATCCAGCAGCATG1434240.27649094770904276No Hit
TTTGCTGTTTCCTTTTATGAGACCCATTCCTATTTCTTATAGTCAATGTT1408110.27145363982219173No Hit
GTTGTTTTAGGGGCTAAAGGGGAGAAACTGAAAGTGTTTTACTCTTTTTC1394250.268781726798397No Hit
TCCACCTGGATGAGAAGTCTTTCCGTTGGTTGCACAACGAGGACCAGATG1357560.26170867565532135No Hit
CCTGAAGAGACAGCAGAAGACACAACAGAAGACACAGAGCAAGACGAAGA1278710.2465080737847432No Hit
TCAGACTAGTGACAAGCTCCTGGTCTTGAGATGTCTTCTCGTTAAGGAGA1253180.24158643312835945No Hit
AAAACTACTAGGATCACGCGGCATGTATTGAGCATATAGGTTGCTGTAGA1250960.2411584643756304No Hit
GTTTTAGGGGCTAAAGGGGAGAAACTGAAAGTGTTTTACTCTTTTTCTAA1230150.23714673926558943No Hit
AGCATGAGAAACCACCACAGTAGCTTTAGAATAGATGCGGTCATATTCTT1207730.23282464042046117No Hit
CGCGGCATGTATTGAGCATATAGGTTGCTGTAGATGAATGTTCTTAGCTG1075690.20737013856895656No Hit
CACCAGCCTGGATAGTCCTGTCACCGTGGAGTGGAAGGCACAGTCTGATT1072480.20675131888595646No Hit
CTCTTCTACAGTACCACCGTCACCTTGTTCAAGGTGAAATGATCCCAACA1071350.20653347893524304No Hit
ACAGCATGCCTTCAAATCAATCTGTAAAACTAAGAAACTTAAATTTTAGT1056520.20367457055552618No Hit
CTGCACCACACATTCAAAAGGATCGTTTTGTTTTGTTTTTAAAGAAAGGT1054380.2032620241001928No Hit
CTCTCGATATTCCTGTGCAGAAACCTGGACCACGTCTACAACCGGCTCGC1028570.19828640540292428No Hit
GTGAGGAAGAAGGAGAGGAATACTAAAGTTAAAACGTCACAAAGGTGCTG1021200.1968656262553509No Hit
TCCCCGACTCCTGCTGCATTAATGTTACTGTGGGCTGTGGGATTAATTTC975050.1879688884452408No Hit
AACACAATTAGGCTGGCTAACGGATAGTGAGCTTGTGCCCCTGCCTAGGT969880.18697222247604753No Hit
GGATGGAGTTCACAGGCGAATAGAGGAGAGGACCAGGGGACGTGGCTTGT967680.1865481092976674No Hit
GGAGTGCTGCTGTAGATGACAACCTCCATTCTATTTTAGAATAAATTCCC956030.18430223723942726No Hit
TGGTGGAGATGACTGAAGCCCGACACGGCCTGAGCGTCCAGAAATGGTGC954910.18408632507588832No Hit
TGGAGACGCATTTCGGTTGAGAATGGAAACCACAACTTTTAACCAAACAA904750.17441654460882172No Hit
GTGCATTTCAATCCCACGTACTTATAAAGAAGGTTGGTGAATTTCACAAG898480.17320782205043841No Hit
AGCAGGCTGAAGAGGAACGATATTTCCGAGCACAGAGTAGAGAACAACTG890600.1716887257569678No Hit
AGCCCACTGAACCTCTGCCAATTAAGATGGTGTTGGGTTAGGTTACATCT871750.16805484693312No Hit
TACGCCGACTGTCATGCTTCAAACCTCAATGGTCTCTACCTCATGGGACC846900.1632642958045992No Hit
TTCTACACAGAAGATTCACCTGGGCTCAAAGTTGGCCCTGTTCCAGTATT810340.1562163058947915No Hit
AGAAGGCCTTAGGTGTTGCATGTCTATGCTTGAGGAACTTTTCCAAATGT737830.14223792109281783No Hit
CTGAGGAACGAAAGAAACTTCGACAAGAGAATGGAAATGTACATGCTATA737370.1421492428827929No Hit
TCAACGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTAC717140.1382493294288703No Hit
ATTTGCAATTTTTGACCCTGACCACTAACTAGTGATTCTTCTCCAAAATT715580.13794859462965534No Hit
TTGCTACCGGTTCAGCAACTCTGCAGAAAATGTCCATCCTTGTGGCTTCC708300.13654516556665203No Hit
GCTCTTGAGGATGTCACCAATTAACCAGAAATCCAGTTATTTTCCACCCT671420.12943548646726175No Hit
AGAGGCCGTATTACAAATGAGCCCGAATCATGACAGTCAGCAACATGATA657770.1268040569741306No Hit
CACATCCTAAGCCAAACCCCAAAAACAACTGATATAATTACTCAAGAAAT648680.12505169843255096No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG631290.12169927653617359No Hit
GCAACTGCAGAAAATGTCCTCCCTTGTGGCTTCCTCAGCTCCTGTTCTTG625800.12064092137739778No Hit
GAAATCTAGCTGCTGAATACGCTGAAGTAAATCCTTGTTCACTGAAGTCT591000.11393222201029414No Hit
TCGCTTAAGGAATCTGCCCCACAGCTTCCCCCATAGAAGGATTTCATGAG579480.11171141118532192No Hit
TCACATGTAGGTGGCGTGTATGAGTGGAGACGGGCCTGGGTCTTGGGGAC567800.10945975576555841No Hit
GGCAAATGGCGACTCTATACTCTCCGCCCGCCCCAGGGCTGGATGGATTA564680.10885828616712843No Hit
GCAACTGCAGAAAATGTCCTCCCTTGTGGCTTCCTCAGCTCCTGCCCTTG552800.10656807500387581No Hit
TAATCTGATTGGTGGCCTAGCTATGGCTTGCCATGACTCCTTCCTCAAGG540790.10425280260735528No Hit
AGCCTGACGAGCTGCCCGAGGTGGATAACCTGACACTGGACGGGAATCCC522260.10068061297309006No Hit
GCAATATGTGATGTGGCAAATCTCTATTAGGAAATATTCTGTAATCTTCA520940.100426145066062No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)