FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005475322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101184965
Sequences flagged as poor quality0
Sequence length93
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTAATACCTGCCACCCCACTCTTAATCAGTGGTGGAAGAACGGTCTC1352792313.369499114814143No Hit
CTCTTCAATCTCTTGCACTCAAAGCTTGTTAAGATAGTTAAGCGTGCATA47627744.7069977244148875No Hit
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG26448772.613903162391764No Hit
CTCATTAAAGCAGCCGGTGTAAATGTTGAGCCTTTTTGGCCTGGCTTGTT26358472.604978911639689No Hit
ATGCTGATGGCGAGGCTAACCTGGGCCTCCCACGAGAAGATGCACGAGGG25574042.5274545482127704No Hit
TGGATCAAGTTTAAATGACTGTGCTGCCCCTTTCACATCAAAGAACTACT25498872.520025578899No Hit
TTGAAGACGTCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCTGCT23653702.3376694353751075No Hit
AGAAACAGACTGCCTGAATTGAGAATTTTGATTTTTTAAAGTGTGTTTCT21230672.09820401677265No Hit
AACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTGTGACTTC16827061.6630000316746663No Hit
TGGGATCTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACAT16202531.6012784112738487No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCACCTGAA15092811.4916059910679418No Hit
ATCTCTGTGACTTCCTGGAGACTCACTTCCTAGATGAGGAAGTGAAGCTT15091301.4914567594108472No Hit
TTGTTGAGGTGGTCTGAATGTTCTGACATTAACAGTTTTCCATGAAAACG13932651.376948640541606No Hit
AAGGCACAGGGAGAAGGGATAACCCTACACCCAGACCCCAGGCTGGACAT13550521.3391831484054968No Hit
TAGAGACCGAGTGTCCTCAGTATATCAGGAAAAAGGGTGCAGACGTCTGG13045861.289308149684096No Hit
ATGTCCACCATGACCCTCTTCCCACGCTGACCTGTGCTCCCTCCCCAATC11091671.0961776781758041No Hit
TCCGTCTTATTTCATTTCTGTAACAGTTGATATCTGGCTGTCCTTTTTAT10336191.021514411750797No Hit
CTCTAGAGGCTGGCAAGAACCAGTTGTTTTGTCTTGCGGGTCTGTCAGGG10140781.0022022540601758No Hit
CAACAGAAAAACAATCTTATTCCGAGCATTCCAGTAACTTTTTTGTGTAT10073980.9956004827397036No Hit
GTTTCAGTGAACAAGTTTCAGCGGTTCAACTTTATAATAATTATAAATAA9271030.9162458078628579No Hit
TAGGTGTCTGCCTTCACAAATGTCATTGTCTACTCCTAGAAGAACCAAAT9008490.8902992653108097No Hit
GACTGTTCACAGACCTCAGTGATGTGAGCACTGTTGCTCAGGAGTGACAA8500070.8400526698803522No Hit
CAAGCTCATTTCCTGGTATGACAACGAATTTGGCTACAGCAACAGGGTGG7561340.7472790053344388No Hit
GTGAGGAAGAAGGAGAGGAATACTAATTATCCATTCCTTTTGGCCCTGCA7516030.7428010673324836No Hit
TTTCCATGTAGAAAGGCAGGAATGTCTCCTAAGCTTTCCTGGACAGCAGA6687490.66091736059799No Hit
ACAATCCTTGACCTCAGTGAAAGCAGTCATCTTCAGCATTTTCCAGCCCT6650970.6573081287323665No Hit
GATGATGATGAAAGTCTTCTTGGACACTTAATGATTGTTGGCAAGAAATG6489230.6413235405082168No Hit
GCATATAGAGGCAAGAAAGAGATACCAAGATGCCATCTGTGTCCTGGTTA6266020.6192639390644648No Hit
GGTAGGACTGTAGAAAAGGGAACTGAACATTCCAGAGCGTGTAGTGAATC6104470.6032981283335919No Hit
ACAATCCTTGACCTCAGTGAAAGCAGTCATCTTCAGCGTTTTCCAGCCCT5764450.5696943216810917No Hit
ATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGAC5633050.5567082026465098No Hit
CTGAATGTAACCTGCTAGCTCTCCGAAGGCCCTGCGGGCCTGGCTTGTGT4747220.4691625875445033No Hit
CGCCATGATGTGTAACGAATTCTTTGAAGGCTTCCCAGATAAGCAGCCCA4692950.46379914249117943No Hit
TGGTATTTTCAATAGCCACTAACCTTGCCTGGTACAGTATGGGGGTTGTA4559090.45056990433311905No Hit
GACTGTCTAAAGGATGCCTGGATTCCTTGTTATCTCAGGACTCTAAATAC4378160.43268878928801335No Hit
GATCTCCTTCGCTGACTACAACCTGCTGGACTTGCTGCTGATCCATGAGG4096270.4048299072890918No Hit
TACCCCCGTCCTCTTCGAGACCCCAGTCGTGATGTGGAGGAAGAGCCACC4039930.3992618863879629No Hit
GCAATAAGAAATGAACAAAAGATTGGAGCTGAAGACCTAAAGTGCTTGCT4000700.3953848281708651No Hit
GGCAGACCTGTTATCCTAAACTAGGGTTTTTAATGACCACAACAAGCAAG3822960.3778189773549855No Hit
CCTTCGATCTCCTGACGAGTTTATTGTTGGCCAAAACCAGGCTTTGATTG3593480.3551397186330993No Hit
AGACGGTTGAAACTAGAGATGGACAGGTTATCAACGAAACTTCTCAGCAT3581410.353946853665463No Hit
TGACCCTTCAAGCTCTGTCCTTATCCATTACCTCAAAGCAGTCATTCCTT3312450.3273658294984833No Hit
TTCCCTCTCCTGTCCTTGTGTTGAAGGCAGTAAACTAAGGGTGTCAAGCC3284120.3245660064220015No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCATCTGAA2842210.2808925219275413No Hit
CTACAAATGAGCTCTTGCAACAGAACTCACAGCCTTTTTACTTTTTTCCC2830370.27972238760966117No Hit
GACAAAACGACACCAGCCAAACCAGCAGCCCCTCAGCATCCAGCAGCATG2828190.27950694058153797No Hit
GAGAACTGCTTCTAAGATGCCAGCATATACTGAAGTCTTTTCTGTCACCA2690540.2659031408470616No Hit
GACAGTGATTCCTGCAACTTGACCTTCAGGCTGGGAGAGGTGGAGAGCCA2660300.2629145545486921No Hit
GTTGTTTTAGGGGCTAAAGGGGAGAAACTGAAAGTGTTTTACTCTTTTTC2616270.25856311755407535No Hit
TCCACCTGGATGAGAAGTCTTTCCGTTGGTTGCACAACGAGGACCAGATG2431780.2403301715823097No Hit
TTTGCTGTTTCCTTTTATGAGACCCATTCCTATTTCTTATAGTCAATGTT2321010.22938289300193956No Hit
AAAACTACTAGGATCACGCGGCATGTATTGAGCATATAGGTTGCTGTAGA2308480.22814456673479108No Hit
TCAGACTAGTGACAAGCTCCTGGTCTTGAGATGTCTTCTCGTTAAGGAGA2305540.22785400973356068No Hit
GTTTTAGGGGCTAAAGGGGAGAAACTGAAAGTGTTTTACTCTTTTTCTAA2294340.22674712591934978No Hit
CTCTTCTACAGTACCACCGTCACCTTGTTCAAGGTGAAATGATCCCAACA2288290.2261492110018519No Hit
AGCATGAGAAACCACCACAGTAGCTTTAGAATAGATGCGGTCATATTCTT2270610.22440191583799038No Hit
CCTGAAGAGACAGCAGAAGACACAACAGAAGACACAGAGCAAGACGAAGA2256790.22303610027438364No Hit
AACACAATTAGGCTGGCTAACGGATAGTGAGCTTGTGCCCCTGCCTAGGT2070870.2046618289584821No Hit
CTGCACCACACATTCAAAAGGATCGTTTTGTTTTGTTTTTAAAGAAAGGT1991310.19679900072110515No Hit
CGCGGCATGTATTGAGCATATAGGTTGCTGTAGATGAATGTTCTTAGCTG1989810.19665075735313045No Hit
GTGAGGAAGAAGGAGAGGAATACTAAAGTTAAAACGTCACAAAGGTGCTG1965530.19425119137018035No Hit
CTCTCGATATTCCTGTGCAGAAACCTGGACCACGTCTACAACCGGCTCGC1956930.19340126272712552No Hit
ACAGCATGCCTTCAAATCAATCTGTAAAACTAAGAAACTTAAATTTTAGT1935040.19123789784381504No Hit
TGGTGGAGATGACTGAAGCCCGACACGGCCTGAGCGTCCAGAAATGGTGC1827980.18065727452690228No Hit
GGATGGAGTTCACAGGCGAATAGAGGAGAGGACCAGGGGACGTGGCTTGT1796350.17753131604087624No Hit
AGCAGGCTGAAGAGGAACGATATTTCCGAGCACAGAGTAGAGAACAACTG1726820.17065974179068993No Hit
GGAGTGCTGCTGTAGATGACAACCTCCATTCTATTTTAGAATAAATTCCC1718180.1698058599911558No Hit
CACCAGCCTGGATAGTCCTGTCACCGTGGAGTGGAAGGCACAGTCTGATT1708180.16881757087132462No Hit
TCCCCGACTCCTGCTGCATTAATGTTACTGTGGGCTGTGGGATTAATTTC1707250.1687256599831803No Hit
TGGAGACGCATTTCGGTTGAGAATGGAAACCACAACTTTTAACCAAACAA1681100.16614128393482175No Hit
GTGCATTTCAATCCCACGTACTTATAAAGAAGGTTGGTGAATTTCACAAG1669660.16501068118173484No Hit
AGCCCACTGAACCTCTGCCAATTAAGATGGTGTTGGGTTAGGTTACATCT1658420.1638998442110446No Hit
TACGCCGACTGTCATGCTTCAAACCTCAATGGTCTCTACCTCATGGGACC1579060.15605678175606424No Hit
TCAACGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTAC1495740.14782235680963074No Hit
TTCTACACAGAAGATTCACCTGGGCTCAAAGTTGGCCCTGTTCCAGTATT1494980.14774724683652357No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1464060.14469145687800555No Hit
TTGCTACCGGTTCAGCAACTCTGCAGAAAATGTCCATCCTTGTGGCTTCC1370460.1354410707163856No Hit
AGAAGGCCTTAGGTGTTGCATGTCTATGCTTGAGGAACTTTTCCAAATGT1346660.13308894261118734No Hit
CTGAGGAACGAAAGAAACTTCGACAAGAGAATGGAAATGTACATGCTATA1333710.13180910820100594No Hit
GCAACTGCAGAAAATGTCCTCCCTTGTGGCTTCCTCAGCTCCTGTTCTTG1299350.12841334678526597No Hit
GCTCTTGAGGATGTCACCAATTAACCAGAAATCCAGTTATTTTCCACCCT1280710.12657117586590064No Hit
AGAGGCCGTATTACAAATGAGCCCGAATCATGACAGTCAGCAACATGATA1248240.12336220109380874No Hit
TCACATGTAGGTGGCGTGTATGAGTGGAGACGGGCCTGGGTCTTGGGGAC1207890.11937445449528988No Hit
ATTTGCAATTTTTGACCCTGACCACTAACTAGTGATTCTTCTCCAAAATT1197160.11831402026971102No Hit
CACATCCTAAGCCAAACCCCAAAAACAACTGATATAATTACTCAAGAAAT1191680.11777243783204353No Hit
GCAACTGCAGAAAATGTCCTCCCTTGTGGCTTCCTCAGCTCCTGCCCTTG1140300.11269460833435085No Hit
TAATCTGATTGGTGGCCTAGCTATGGCTTGCCATGACTCCTTCCTCAAGG1131200.11179526523530448No Hit
GGCAAATGGCGACTCTATACTCTCCGCCCGCCCCAGGGCTGGATGGATTA1117720.11046305150177202No Hit
AGCCTGACGAGCTGCCCGAGGTGGATAACCTGACACTGGACGGGAATCCC1073960.10613829831339072No Hit
GAAATCTAGCTGCTGAATACGCTGAAGTAAATCCTTGTTCACTGAAGTCT1068420.10559078614100426No Hit
TCGCTTAAGGAATCTGCCCCACAGCTTCCCCCATAGAAGGATTTCATGAG1059730.10473196289587094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)