FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005475324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102410193
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTAATACCTGCCACCCCACTCTTAATCAGTGGTGGAAGAACGGTCTC1424021613.905076812031785No Hit
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG99618479.727397935867575No Hit
CTCATTAAAGCAGCCGGTGTAAATGTTGAGCCTTTTTGGCCTGGCTTGTT50749834.955544805974538No Hit
CTCTTCAATCTCTTGCACTCAAAGCTTGTTAAGATAGTTAAGCGTGCATA44200864.316060609318449No Hit
TTGAAGACGTCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCTGCT34477763.3666336318690466No Hit
TGGATCAAGTTTAAATGACTGTGCTGCCCCTTTCACATCAAAGAACTACT24867372.4282123948345653No Hit
TGGGATCTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACAT21417112.09130647766673No Hit
ATCTCTGTGACTTCCTGGAGACTCACTTCCTAGATGAGGAAGTGAAGCTT19336051.8880981896011073No Hit
CTCTAGAGGCTGGCAAGAACCAGTTGTTTTGTCTTGCGGGTCTGTCAGGG15796141.5424382610039609No Hit
GACTGTTCACAGACCTCAGTGATGTGAGCACTGTTGCTCAGGAGTGACAA15669981.5301191747583174No Hit
AACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTGTGACTTC14618671.427462401130325No Hit
ATGTCCACCATGACCCTCTTCCCACGCTGACCTGTGCTCCCTCCCCAATC14409421.4070298646932538No Hit
CAAGCTCATTTCCTGGTATGACAACGAATTTGGCTACAGCAACAGGGTGG13710731.3388052105321195No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCACCTGAA10461411.021520387135683No Hit
CAACAGAAAAACAATCTTATTCCGAGCATTCCAGTAACTTTTTTGTGTAT8964920.8753933312087401No Hit
GATGATGATGAAAGTCTTCTTGGACACTTAATGATTGTTGGCAAGAAATG7487500.731128394612048No Hit
GGCAGACCTGTTATCCTAAACTAGGGTTTTTAATGACCACAACAAGCAAG7251220.708056472464611No Hit
TTTCCATGTAGAAAGGCAGGAATGTCTCCTAAGCTTTCCTGGACAGCAGA7198890.7029466295410653No Hit
GTTTCAGTGAACAAGTTTCAGCGGTTCAACTTTATAATAATTATAAATAA6816070.6655655848632177No Hit
TACCCCCGTCCTCTTCGAGACCCCAGTCGTGATGTGGAGGAAGAGCCACC6605200.6449748610472787No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCATCTGAA6378800.6228676866178741No Hit
AGCCCACTGAACCTCTGCCAATTAAGATGGTGTTGGGTTAGGTTACATCT6279240.6131459980746252No Hit
AAGGCACAGGGAGAAGGGATAACCCTACACCCAGACCCCAGGCTGGACAT5873990.5735747417251719No Hit
TCCGTCTTATTTCATTTCTGTAACAGTTGATATCTGGCTGTCCTTTTTAT5616920.5484727482156No Hit
TTCCCTCTCCTGTCCTTGTGTTGAAGGCAGTAAACTAAGGGTGTCAAGCC5312920.5187882030453745No Hit
ATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGAC5281610.5157308901859017No Hit
CCTTCGATCTCCTGACGAGTTTATTGTTGGCCAAAACCAGGCTTTGATTG5115260.49948738989291813No Hit
GATCTCCTTCGCTGACTACAACCTGCTGGACTTGCTGCTGATCCATGAGG5020230.49020804013131775No Hit
CTGAGGAACGAAAGAAACTTCGACAAGAGAATGGAAATGTACATGCTATA4993950.48764188931857594No Hit
AGACGGTTGAAACTAGAGATGGACAGGTTATCAACGAAACTTCTCAGCAT4912260.47966514426937956No Hit
TAGGTGTCTGCCTTCACAAATGTCATTGTCTACTCCTAGAAGAACCAAAT4790110.4677376206096985No Hit
GTGAGGAAGAAGGAGAGGAATACTAATTATCCATTCCTTTTGGCCCTGCA4537300.4430516013186304No Hit
ACAGCATGCCTTCAAATCAATCTGTAAAACTAAGAAACTTAAATTTTAGT4499850.43939473876394314No Hit
CTACAAATGAGCTCTTGCAACAGAACTCACAGCCTTTTTACTTTTTTCCC4320870.42191796279497296No Hit
TTGTTGAGGTGGTCTGAATGTTCTGACATTAACAGTTTTCCATGAAAACG4236250.4136551134123924No Hit
TGGTATTTTCAATAGCCACTAACCTTGCCTGGTACAGTATGGGGGTTGTA3896750.38050411642130194No Hit
GCCAATGAATGTACTCTGTGCATGAAAATCAGGGAAGGTGGTCATAATAT3731860.3644031800623596No Hit
CGCCATGATGTGTAACGAATTCTTTGAAGGCTTCCCAGATAAGCAGCCCA3624130.353883719367661No Hit
GCAATAAGAAATGAACAAAAGATTGGAGCTGAAGACCTAAAGTGCTTGCT3557090.34733749598538494No Hit
TTTGCTGTTTCCTTTTATGAGACCCATTCCTATTTCTTATAGTCAATGTT3468030.33864109600887093No Hit
GAGGAATTATGAACTGGGGTAAACCCATTTTGAATATTAGCATTGCCAAT3414340.3333984538043005No Hit
GACAGTGATTCCTGCAACTTGACCTTCAGGCTGGGAGAGGTGGAGAGCCA3166800.30922703172720317No Hit
CTTCAGGCTCTTGTTCCGGATGTGGGTCCCTCTCTCTGCCGCTCCTGGCA3094600.3021769522492746No Hit
GACAAAACGACACCAGCCAAACCAGCAGCCCCTCAGCATCCAGCAGCATG2863810.27964110955244464No Hit
TCAACGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTAC2813500.27472851261983267No Hit
GGTAGGACTGTAGAAAAGGGAACTGAACATTCCAGAGCGTGTAGTGAATC2721170.2657128084896784No Hit
TCCACCTGGATGAGAAGTCTTTCCGTTGGTTGCACAACGAGGACCAGATG2686040.2622824858849744No Hit
CTCTCGATATTCCTGTGCAGAAACCTGGACCACGTCTACAACCGGCTCGC2504820.24458698168843407No Hit
GTGCTCACCACACATCTGGGGCAGCCTTCCCTGGCAGGGGCCCTCGTGGC2501150.24422861892272776No Hit
TCAACGAGATCGAGACCATCCTGGCCAACATGGTGAAACCCTGTCTGTAC2495560.2436827748191042No Hit
GTGAGGAAGAAGGAGAGGAATACTAAAGTTAAAACGTCACAAAGGTGCTG2481340.24229424116015483No Hit
TCACATGTAGGTGGCGTGTATGAGTGGAGACGGGCCTGGGTCTTGGGGAC2261790.22085594546238188No Hit
CTCTGAACCCCAATCCTCAAAATGAAGATACTGACACCACCTTTGCCCTC2201690.21498738899945244No Hit
CCTTCTTGGGCCTGCCTCTTAAAGGCCTGAGCTAGCTGGAGAAGAGGGGA2039180.1991188513823033No Hit
GACTGTCTAAAGGATGCCTGGATTCCTTGTTATCTCAGGACTCTAAATAC2010980.19636521923164424No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1907440.18625489749833787No Hit
AAAACTACTAGGATCACGCGGCATGTATTGAGCATATAGGTTGCTGTAGA1880710.1836448057470217No Hit
TCCAAACCAACCACACCTTGAAGCAGACTCTGAGCTGCTACTCTGAAGTC1798510.17561826096744101No Hit
ACCTGACTCTCTTAAGGAGGCTGAGAGCCCAAACTGCTGTCCCAAACATG1791580.17494157051339607No Hit
CCTGAAGAGACAGCAGAAGACACAACAGAAGACACAGAGCAAGACGAAGA1788770.17466718376363183No Hit
ACAATCCTTGACCTCAGTGAAAGCAGTCATCTTCAGCGTTTTCCAGCCCT1788050.17459687826191284No Hit
ACAATCCTTGACCTCAGTGAAAGCAGTCATCTTCAGCATTTTCCAGCCCT1746430.1705328296764366No Hit
AGGAACTTCAGAAACCCCTTGGCCAAGTAAGCTGTGGGCAGGCAAGCCCT1724740.16841487643715308No Hit
GCTGTGCACATTCCCTCCTGCTCCCCAGAGACTGCCTCCGCCATCCCACA1715420.16750481077601329No Hit
CCCGTCCTGGCTATGAAACCTTGTGAGCAGAAGGCAAGAGCGGCAAGATG1708080.1667880852446006No Hit
CTGAATGTAACCTGCTAGCTCTCCGAAGGCCCTGCGGGCCTGGCTTGTGT1635760.15972628818305226No Hit
CGCAACTTTGGCAAGGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAA1634740.15962668872228372No Hit
TGACCCTTCAAGCTCTGTCCTTATCCATTACCTCAAAGCAGTCATTCCTT1590890.15534488837453905No Hit
TCAGACTAGTGACAAGCTCCTGGTCTTGAGATGTCTTCTCGTTAAGGAGA1583850.15465745680217594No Hit
GCAACGACCTGGAGAGACAAGGCCTACTTTCGGGCGCTGGCGCCTCTACA1573360.15363314470074282No Hit
GACAAAACGACACCAGCCAAACCAGCAGCCCCTCAGCATCCAGCAACATA1568650.15317322954366466No Hit
CGCGGCATGTATTGAGCATATAGGTTGCTGTAGATGAATGTTCTTAGCTG1559380.15226804620903311No Hit
TCCCCGACTCCTGCTGCATTAATGTTACTGTGGGCTGTGGGATTAATTTC1548630.15121834600975706No Hit
ATGTGACGGCAGGGAAATGTATGTGTGTCTATTTTGTAACTGTAAAGATG1517040.1481336921218379No Hit
TAGAGACCGAGTGTCCTCAGTATATCAGGAAAAAGGGTGCAGACGTCTGG1513130.14775189418889192No Hit
AACACAATTAGGCTGGCTAACGGATAGTGAGCTTGTGCCCCTGCCTAGGT1468870.1434300587637795No Hit
GGATTACTTTTGATCCTGGGGGACCAATGAGGTGAGGGGGGTTCTCCTTT1401430.13684477676943738No Hit
ACCTAAATTAAGACTCTGACTTGGATTGTGAATTATAATGATATGCCCCT1363210.13311272638652288No Hit
GAAGCTGAGCTGAACACATTACGATGGATGATGGAAACATAAGACTATCA1237580.1208453928018669No Hit
GCAACTGCAGAAAATGTCCTCCCTTGTGGCTTCCTCAGCTCCTGTTCTTG1201040.11727738858962995No Hit
GCTGACCTCACCCTTACTTTTATACTTTAGTATGAAACTGATGAGAACTT1195490.11673545034721299No Hit
AGCAGGCTGAAGAGGAACGATATTTCCGAGCACAGAGTAGAGAACAACTG1188660.11606852454618458No Hit
GCCAATCCTCTTACACCTTTGACCATAGAATCAAGCCTGAGGCTGTGAAG1186390.11584686692270954No Hit
TTCTACACAGAAGATTCACCTGGGCTCAAAGTTGGCCCTGTTCCAGTATT1179650.115188729309396No Hit
AGCGAGAGAAAAGGAAAAGCCAACAGGCCCCCCAGCCAAGAAAGCTATCT1177520.11498074220014408No Hit
TGGAGACGCATTTCGGTTGAGAATGGAAACCACAACTTTTAACCAAACAA1124220.10977618214233811No Hit
GGGTTTGCTTTGGAAATCCTCTAGGAGGCTCCTCCTCGCATGGCCTGCAG1110640.1084501422626945No Hit
AGAGGCCGTATTACAAATGAGCCCGAATCATGACAGTCAGCAACATGATA1098210.10723639589274087No Hit
ATGCTGATGGCGAGGCTAACCTGGGCCTCCCACGAGAAGATGCACGAGGG1082400.10569260425082883No Hit
TATTGCAGACTTGGAAGGAAGCATAAGCTTCCCATTTCACAAAGGGGAAA1065180.10401113100138382No Hit
TATCGAATCCAGTGTTCTGTCAGCGCGCTCAACCAGGGTCAAGTCTGGAT1054800.1029975600182689No Hit
GGCTCTTTCCCTCATGTATACTTCAAGTAAGATCAAGAATCTTTTGTGAA1054550.10297314838572758No Hit
CCTAACAGGCTGTAGATTACCAAAACAAGATTAATTTCATCACTAAAAGA1053090.10283058445168637No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)