FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005475328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90858227
Sequences flagged as poor quality0
Sequence length77
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAAGACGTCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCTGCT57141636.289098069237033No Hit
TCCCTAATACCTGCCACCCCACTCTTAATCAGTGGTGGAAGAACGGTCTC52277665.75376184701469No Hit
CTCTTCAATCTCTTGCACTCAAAGCTTGTTAAGATAGTTAAGCGTGCATA35191433.873224380660653No Hit
TGGATCAAGTTTAAATGACTGTGCTGCCCCTTTCACATCAAAGAACTACT34885693.8395741532574696No Hit
TACCCCCGTCCTCTTCGAGACCCCAGTCGTGATGTGGAGGAAGAGCCACC29458743.24227546284829No Hit
ATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGAC27226542.9965960044542803No Hit
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG21668312.384848429851047No Hit
CTCATTAAAGCAGCCGGTGTAAATGTTGAGCCTTTTTGGCCTGGCTTGTT13883121.5279981195318724No Hit
GTGAGGAAGAAGGAGAGGAATACTAATTATCCATTCCTTTTGGCCCTGCA11356481.2499121295862399No Hit
GACAAAACGACACCAGCCAAACCAGCAGCCCCTCAGCATCCAGCAGCATG11315661.245419415899454No Hit
ATGTCCACCATGACCCTCTTCCCACGCTGACCTGTGCTCCCTCTCCAATC11303961.2441316954159805No Hit
ATGTCCACCATGACCCTCTTCCCACGCTGACCTGTGCTCCCTCCCCAATC8590420.9454751962087042No Hit
GCAACTGCAGAAAATGTCCTCCCTTGTGGCTTCCTCAGCTCCTGTTCTTG8184730.9008243139061034No Hit
CAACAGAAAAACAATCTTATTCCGAGCATTCCAGTAACTTTTTTGTGTAT7826110.8613540301639389No Hit
AGACGGTTGAAACTAGAGATGGACAGGTTATCAACGAAACTTCTCAGCAT7215890.7941922529481011No Hit
CTCTAGAGGCTGGCAAGAACCAGTTGTTTTGTCTTGCGGGTCTGTCAGGG6625480.7292107956277861No Hit
TGGTATTTTCAATAGCCACTAACCTTGCCTGGTACAGTATGGGGGTTGTA6408630.7053439420516097No Hit
GTTTCAGTGAACAAGTTTCAGCGGTTCAACTTTATAATAATTATAAATAA5942340.6540233280140939No Hit
TGACCCTTCAAGCTCTGTCCTTATCCGTTACCTTAAAGCAGTCATTCCTT5935410.6532606012661902No Hit
TTTCCATGTAGAAAGGCAGGAATGTCTCCTAAGCTTTCCTGGACAGCAGA5898540.6492026308195514No Hit
CGCCATGATGTGTAACGAATTCTTTGAAGGCTTCCCAGATAAGCAGCCCA4734030.5210348205451995No Hit
TTCCCTCTCCTGTCCTTGTGTTGAAGGCAGTAAACTAAGGGTGTCAAGCC3977100.43772590895924046No Hit
GATGATGATGAAAGTCTTCTTGGACACTTAATGATTGTTGGCAAGAAATG3976470.4376565701639764No Hit
TGGGATCTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACAT3543170.38996688764353726No Hit
CTTCAGGCTCTTGTTCCGGATGTGGGTCCCTCTCTCTGCCGCTCCTGGCA3427380.3772228573203393No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCACCTGAA3336290.367197348017808No Hit
TCCGTCTTATTTCATTTCTGTAACAGTTGATATCTGGCTGTCCTTTTTAT3333760.3669188922209543No Hit
GCTGGCCATTATTAAGTCCCTGTAACTCAAATGTCAACCCCACCGAGGCA3248720.35755925547611667No Hit
AAGGCACAGGGAGAAGGGATAACCCTACACCCAGACCCCAGGCTGGACAT3168340.34871250569307277No Hit
AGCCCACTGAACCTCTGCCAATTAAGATGGTGTTGGGTTAGGTTACATCT3042940.3349107835881499No Hit
CCTGTGCTGGCGCTGGAAAATTATTTGTGCTCAGCTGACATTTAACACTC2667960.29363989240071786No Hit
GACTGTCTAAAGGATGCCTGGATTCCTTGTTATCTCAGGACTCTAAATAC2630700.2895389979379633No Hit
ATCTCTGTGACTTCCTGGAGACTCACTTCCTAGATGAGGAAGTGAAGCTT2519040.2772495219392736No Hit
CTCTGAACCCCAATCCTCAAAATGAAGATACTGACACCACCTTTGCCCTC2422280.2665999634793666No Hit
TCGCTTAAGGAATCTGCCCCACAGCTTCCCCCATAGAAGGATTTCATGAG2417300.2660518568120419No Hit
GGTACGGGCCAGGAAGAATATGTGGCAGAGCTCCTGGAAATGATGCAGAT2371620.2610242438475054No Hit
AGAAACAGACTGCCTGAATTGAGAATTTTGATTTCTTAAAGTGTGTTTCT2364400.2602295992414644No Hit
GGTAGGACTGTAGAAAAGGGAACTGAACATTCCAGAGCGTGTAGTGAATC2350730.2587250574458161No Hit
CTCTTCTACAGTACCACCGTCACCTTGTTCAAGGTGAAATGATCCCAACA2297020.2528136499956135No Hit
AACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTGTGACTTC2265620.24935771639039359No Hit
GCCTATCACCTCTTATGCACACCTCCCACCCCCTGTATTCCCACCCCTGG2129430.23436842983960054No Hit
TTTGCTGTTTCCTTTTATGAGACCCATTCCTATTTCTTATAGTCAATGTT2122890.2336486271078127No Hit
GATCTCCTTCGCTGACTACAACCTGCTGGACTTGCTGCTGATCCATGAGG2072930.22814995058180035No Hit
GGCAGACCTGTTATCCTAAACTAGGGTTTTTAATGACCACAACAAGCAAG2066120.2274004312234708No Hit
AAAACTACTAGGATCACGCGGCATGTATTGAGCATATAGGTTGCTGTAGA2031920.2236363251948555No Hit
ACAGCATGCCTTCAAATCAATCTGTAAAACTAAGAAACTTAAATTTTAGT2031100.22354607469943255No Hit
AGCAGGCTGAAGAGGAACGATATTTCCGAGCACAGAGTAGAGAACAACTG1922760.21162200314562596No Hit
GCAACTGCAGAAAATGTCCTCCCTTGTGGCTTCCTCAGTTCCTGCCCTTG1887510.20774233245823737No Hit
TAATCTGATTGGTGGCCTAGCTATGGCTTGCCATGACTCCTTCCTCAAGG1868840.20568748276366872No Hit
GGCCATGATTATCTTAAAGGCATTATTCTCCAGCCTTAAGATCTTAGGAC1847420.20332996372469386No Hit
CCTTCGATCTCCTGACGAGTTTATTGTTGGCCAAAACCAGGCTTTGATTG1837280.20221393930568338No Hit
GACTGTTCACAGACCTCAGTGATGTGAGCACTGTTGCTCAGGAGTGACAA1820690.20038801769706555No Hit
CTGAATGTAACCTGCTAGCTCTCCGAAGGCCCTGCGGGCCTGGCTTGTGT1772460.19507974770407968No Hit
CGCGGCATGTATTGAGCATATAGGTTGCTGTAGATGAATGTTCTTAGCTG1718640.18915623348010083No Hit
GCAATAAGAAATGAACAAAAGATTGGAGCTGAAGACCTAAAGTGCTTGCT1644460.18099186549171822No Hit
GTTGTTTTAGGGGCTAAAGGGGAGAAACTGAAAGTGTTTTACTCTTTTTC1614080.17764819469787804No Hit
ACAATCCTTGACCTCAGTGAAAGCAGTCATCTTCAGCATTTTCCAGCCCT1478460.16272164324756194No Hit
GACAGTGATTCCTGCAACTTGACCTTCAGGCTGGGAGAGGTGGAGAGCCA1471950.16200514236316763No Hit
CCCGTCCTGGCTATGAAACCTTGTGAGCAGAAGGCAAGAGCGGCAAGATG1420030.1562907451407785No Hit
ATTTGCAATTTTTGACCCTGACCACTAACTAGTGATTCTTCTCCAAAATT1371390.15093734990008115No Hit
GTTTTAGGGGCTAAAGGGGAGAAACTGAAAGTGTTTTACTCTTTTTCTAA1359860.14966833988517078No Hit
ATGGCCACAGTAGTCTTGATGACCAAAGTCCTTTTTTTCCATCTTTAGAA1337090.1471622377134874No Hit
TGGAGATGACCACGCCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGC1334650.14689368745881426No Hit
CTACAAATGAGCTCTTGCAACAGAACTCACAGCCTTTTTACTTTTTTCCC1309320.14410582764288365No Hit
ACAATCCTTGACCTCAGTGAAAGCAGTCATCTTCAGCGTTTTCCAGCCCT1295250.14255726121532175No Hit
GAGCAGTGAATCAAGGCAGACTTATGAAATCTGTATTATATTTGTAACAG1236770.1361208600295491No Hit
ACCTAAATTAAGACTCTGACTTGGATTGTGAATTATAATGATATGCCCCT1217860.13403959555583225No Hit
GTGAGGAAGAAGGAGAGGAATACTAAAGTTAAAACGTCACAAAGGTGCTG1179680.12983744443967632No Hit
TGGGCATTTGCTACATGATGAGTGCTGCCAGATTGTGGCAGGTAAAGAGA1169970.12876874649997297No Hit
AAAACTACTAGGATCACGCGGCATGTATTGAGGTGTGGCATGCAGCATTT1157750.12742379399501158No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCGTCTGAA1135270.1249496096814656No Hit
CCTGATGTGCATATCCGTACTTCCATGAGGTGTTTTCTGTGTGCAGAACA1111850.1223719674829226No Hit
TCAACGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTAC1109220.1220825055280905No Hit
AGAAACAGACTGCCTGAATTGAGAATTTTGATTTTTTAAAGTGTGTTTCT1078100.11865738916521011No Hit
CTCTCGATATTCCTGTGCAGAAACCTGGACCACGTCTACAACCGGCTCGC1076510.11848239125335341No Hit
ATATATTGGCAGCAACTCCGAGAGAAATGCTTGTTATTTTCTCACACTGT1064470.11715724983275316No Hit
TCAACGAGATCGAGACCATCCTGGCCAACATGGTGAAACCCTGTCTGTAC1025390.11285604329479156No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCATCTGAA1018770.112127435636621No Hit
CCTGAAGAGACAGCAGAAGACACAACAGAAGACACAGAGCAAGACGAAGA1015780.11179835151306661No Hit
GCAATATGTGATGTGGCAAATCTCTATTAGGAAATATTCTGTAATCTTCA1000810.11015072966369902No Hit
TTGTTGAGGTGGTCTGAATGTTCTGACATTAACAGTTTTCCATGAAAACG963100.1060003074900416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)