FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005475340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90824894
Sequences flagged as poor quality0
Sequence length77
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAAGACGTCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCTGCT52670985.79917880223455No Hit
TCCCTAATACCTGCCACCCCACTCTTAATCAGTGGTGGAAGAACGGTCTC50817315.595086078492974No Hit
TGGATCAAGTTTAAATGACTGTGCTGCCCCTTTCACATCAAAGAACTACT37088754.083544540112538No Hit
CTCTTCAATCTCTTGCACTCAAAGCTTGTTAAGATAGTTAAGCGTGCATA34053323.7493377091086946No Hit
TACCCCCGTCCTCTTCGAGACCCCAGTCGTGATGTGGAGGAAGAGCCACC26781802.9487290125546415No Hit
ATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGAC21508462.3681238758175702No Hit
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG21141892.327763795683593No Hit
CTCATTAAAGCAGCCGGTGTAAATGTTGAGCCTTTTTGGCCTGGCTTGTT12929371.4235491428154048No Hit
GTGAGGAAGAAGGAGAGGAATACTAATTATCCATTCCTTTTGGCCCTGCA10892881.1993275764241464No Hit
ATGTCCACCATGACCCTCTTCCCACGCTGACCTGTGCTCCCTCTCCAATC10499021.1559628134550863No Hit
GACAAAACGACACCAGCCAAACCAGCAGCCCCTCAGCATCCAGCAGCATG10117931.114004052677452No Hit
CAACAGAAAAACAATCTTATTCCGAGCATTCCAGTAACTTTTTTGTGTAT8149610.8972881377654016No Hit
ATGTCCACCATGACCCTCTTCCCACGCTGACCTGTGCTCCCTCCCCAATC8097180.8915154913365493No Hit
GCAACTGCAGAAAATGTCCTCCCTTGTGGCTTCCTCAGCTCCTGTTCTTG7701260.8479239183037196No Hit
CTCTAGAGGCTGGCAAGAACCAGTTGTTTTGTCTTGCGGGTCTGTCAGGG6746120.7427611200955544No Hit
AGACGGTTGAAACTAGAGATGGACAGGTTATCAACGAAACTTCTCAGCAT6699350.7376116508322046No Hit
TGGTATTTTCAATAGCCACTAACCTTGCCTGGTACAGTATGGGGGTTGTA6202650.6829240009903011No Hit
GTTTCAGTGAACAAGTTTCAGCGGTTCAACTTTATAATAATTATAAATAA5852990.6444257452147426No Hit
TGACCCTTCAAGCTCTGTCCTTATCCGTTACCTTAAAGCAGTCATTCCTT5614590.6181774349221921No Hit
TTTCCATGTAGAAAGGCAGGAATGTCTCCTAAGCTTTCCTGGACAGCAGA5522300.6080161238613723No Hit
CGCCATGATGTGTAACGAATTCTTTGAAGGCTTCCCAGATAAGCAGCCCA5067160.5579043120050324No Hit
TTCCCTCTCCTGTCCTTGTGTTGAAGGCAGTAAACTAAGGGTGTCAAGCC3742530.41205993590259515No Hit
GATGATGATGAAAGTCTTCTTGGACACTTAATGATTGTTGGCAAGAAATG3697730.40712736752547163No Hit
TGGGATCTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACAT3303150.363683331136065No Hit
TCCGTCTTATTTCATTTCTGTAACAGTTGATATCTGGCTGTCCTTTTTAT3229040.3555236739389974No Hit
GCTGGCCATTATTAAGTCCCTGTAACTCAAATGTCAACCCCACCGAGGCA3098240.3411223359093598No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCACCTGAA2958090.32569154443494314No Hit
CTTCAGGCTCTTGTTCCGGATGTGGGTCCCTCTCTCTGCCGCTCCTGGCA2952320.32505625605244304No Hit
AAGGCACAGGGAGAAGGGATAACCCTACACCCAGACCCCAGGCTGGACAT2915790.32103423098957873No Hit
AGCCCACTGAACCTCTGCCAATTAAGATGGTGTTGGGTTAGGTTACATCT2873850.3164165542543876No Hit
CCTGTGCTGGCGCTGGAAAATTATTTGTGCTCAGCTGACATTTAACACTC2577680.2838076529987472No Hit
GGTACGGGCCAGGAAGAATATGTGGCAGAGCTCCTGGAAATGATGCAGAT2338550.25747896826612315No Hit
AGAAACAGACTGCCTGAATTGAGAATTTTGATTTCTTAAAGTGTGTTTCT2329140.2564429087029818No Hit
GACTGTCTAAAGGATGCCTGGATTCCTTGTTATCTCAGGACTCTAAATAC2302230.25348006461752653No Hit
CTCTGAACCCCAATCCTCAAAATGAAGATACTGACACCACCTTTGCCCTC2273820.25035206757301587No Hit
TCGCTTAAGGAATCTGCCCCACAGCTTCCCCCATAGAAGGATTTCATGAG2267520.24965842514498285No Hit
GGTAGGACTGTAGAAAAGGGAACTGAACATTCCAGAGCGTGTAGTGAATC2259990.24882935729052436No Hit
ATCTCTGTGACTTCCTGGAGACTCACTTCCTAGATGAGGAAGTGAAGCTT2207670.2430688220786693No Hit
GGCAGACCTGTTATCCTAAACTAGGGTTTTTAATGACCACAACAAGCAAG2011930.22151746194165664No Hit
ACAGCATGCCTTCAAATCAATCTGTAAAACTAAGAAACTTAAATTTTAGT2011460.22146571401448595No Hit
AAAACTACTAGGATCACGCGGCATGTATTGAGCATATAGGTTGCTGTAGA2003850.22062783800221117No Hit
CTCTTCTACAGTACCACCGTCACCTTGTTCAAGGTGAAATGATCCCAACA1984820.21853259746166068No Hit
AACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTGTGACTTC1946230.2142837623350268No Hit
GATCTCCTTCGCTGACTACAACCTGCTGGACTTGCTGCTGATCCATGAGG1911370.21044560756657751No Hit
TTTGCTGTTTCCTTTTATGAGACCCATTCCTATTTCTTATAGTCAATGTT1876890.20664929154775563No Hit
GCCTATCACCTCTTATGCACACCTCCCACCCCCTGTATTCCCACCCCTGG1865840.2054326647493803No Hit
GCAACTGCAGAAAATGTCCTCCCTTGTGGCTTCCTCAGTTCCTGCCCTTG1772740.19518217109067035No Hit
AGCAGGCTGAAGAGGAACGATATTTCCGAGCACAGAGTAGAGAACAACTG1771420.19503683648670153No Hit
GGCCATGATTATCTTAAAGGCATTATTCTCCAGCCTTAAGATCTTAGGAC1745840.19222042802494213No Hit
TAATCTGATTGGTGGCCTAGCTATGGCTTGCCATGACTCCTTCCTCAAGG1733070.19081442583351652No Hit
GACTGTTCACAGACCTCAGTGATGTGAGCACTGTTGCTCAGGAGTGACAA1715090.1888347923643049No Hit
CGCGGCATGTATTGAGCATATAGGTTGCTGTAGATGAATGTTCTTAGCTG1679670.18493498049114157No Hit
CCTTCGATCTCCTGACGAGTTTATTGTTGGCCAAAACCAGGCTTTGATTG1664980.1833175825121249No Hit
GCAATAAGAAATGAACAAAAGATTGGAGCTGAAGACCTAAAGTGCTTGCT1557000.1714287714995847No Hit
GTTGTTTTAGGGGCTAAAGGGGAGAAACTGAAAGTGTTTTACTCTTTTTC1498510.16498890711614814No Hit
CTGAATGTAACCTGCTAGCTCTCCGAAGGCCCTGCGGGCCTGGCTTGTGT1484870.16348711620847034No Hit
GACAGTGATTCCTGCAACTTGACCTTCAGGCTGGGAGAGGTGGAGAGCCA1374060.15128671661317875No Hit
ATGGCCACAGTAGTCTTGATGACCAAAGTCCTTTTTTTCCATCTTTAGAA1360120.14975189511368986No Hit
ACAATCCTTGACCTCAGTGAAAGCAGTCATCTTCAGCATTTTCCAGCCCT1319740.14530597745591645No Hit
CCCGTCCTGGCTATGAAACCTTGTGAGCAGAAGGCAAGAGCGGCAAGATG1308330.1440497139473678No Hit
CTACAAATGAGCTCTTGCAACAGAACTCACAGCCTTTTTACTTTTTTCCC1285620.14154929814726788No Hit
GTTTTAGGGGCTAAAGGGGAGAAACTGAAAGTGTTTTACTCTTTTTCTAA1280510.14098667706675222No Hit
ATTTGCAATTTTTGACCCTGACCACTAACTAGTGATTCTTCTCCAAAATT1211870.1334292776603736No Hit
GAGCAGTGAATCAAGGCAGACTTATGAAATCTGTATTATATTTGTAACAG1209470.13316503292588483No Hit
TGGAGATGACCACGCCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGC1192430.13128889531101462No Hit
ACAATCCTTGACCTCAGTGAAAGCAGTCATCTTCAGCGTTTTCCAGCCCT1162740.12801996774144322No Hit
GTGAGGAAGAAGGAGAGGAATACTAAAGTTAAAACGTCACAAAGGTGCTG1129920.12440642099730938No Hit
AAAACTACTAGGATCACGCGGCATGTATTGAGGTGTGGCATGCAGCATTT1119450.12325365334310216No Hit
TGGGCATTTGCTACATGATGAGTGCTGCCAGATTGTGGCAGGTAAAGAGA1111370.12236402940365666No Hit
AGAAACAGACTGCCTGAATTGAGAATTTTGATTTTTTAAAGTGTGTTTCT1091360.1201608889298566No Hit
ACCTAAATTAAGACTCTGACTTGGATTGTGAATTATAATGATATGCCCCT1069350.11773754451064924No Hit
TCAACGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTAC1014530.1117017543670351No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCGTCTGAA1004190.11056329996927934No Hit
GCAATATGTGATGTGGCAAATCTCTATTAGGAAATATTCTGTAATCTTCA992940.10932465277636327No Hit
CTCTCGATATTCCTGTGCAGAAACCTGGACCACGTCTACAACCGGCTCGC964740.1062197771461203No Hit
TCAACGAGATCGAGACCATCCTGGCCAACATGGTGAAACCCTGTCTGTAC951400.10475101683025362No Hit
TTGTTGAGGTGGTCTGAATGTTCTGACATTAACAGTTTTCCATGAAAACG945680.10412123354638872No Hit
ATATATTGGCAGCAACTCCGAGAGAAATGCTTGTTATTTTCTCACACTGT930160.10241245093002806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)