FastQCFastQC Report
Thu 16 May 2024
EGAF00005475350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89538327
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length77
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAAGACGTCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCTGCT54762126.116053519740211No Hit
TCCCTAATACCTGCCACCCCACTCTTAATCAGTGGTGGAAGAACGGTCTC51887405.794993243507889No Hit
TGGATCAAGTTTAAATGACTGTGCTGCCCCTTTCACATCAAAGAACTACT37217504.156599888224402No Hit
CTCTTCAATCTCTTGCACTCAAAGCTTGTTAAGATAGTTAAGCGTGCATA34688493.8741498933747107No Hit
TACCCCCGTCCTCTTCGAGACCCCAGTCGTGATGTGGAGGAAGAGCCACC28054583.133248178738028No Hit
ATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGAC24746042.763737142419469No Hit
AAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGG21246762.372923496772505No Hit
CTCATTAAAGCAGCCGGTGTAAATGTTGAGCCTTTTTGGCCTGGCTTGTT13627291.5219504827245656No Hit
GTGAGGAAGAAGGAGAGGAATACTAATTATCCATTCCTTTTGGCCCTGCA11023751.231176678116847No Hit
GACAAAACGACACCAGCCAAACCAGCAGCCCCTCAGCATCCAGCAGCATG10891091.2163606764732158No Hit
ATGTCCACCATGACCCTCTTCCCACGCTGACCTGTGCTCCCTCTCCAATC10628191.186998948506152No Hit
ATGTCCACCATGACCCTCTTCCCACGCTGACCTGTGCTCCCTCCCCAATC8336630.9310683234007711No Hit
CAACAGAAAAACAATCTTATTCCGAGCATTCCAGTAACTTTTTTGTGTAT8002280.8937267724468428No Hit
GCAACTGCAGAAAATGTCCTCCCTTGTGGCTTCCTCAGCTCCTGTTCTTG7827510.8742077568637171No Hit
CTCTAGAGGCTGGCAAGAACCAGTTGTTTTGTCTTGCGGGTCTGTCAGGG6995840.781323510768746No Hit
AGACGGTTGAAACTAGAGATGGACAGGTTATCAACGAAACTTCTCAGCAT6920600.7729204053589253No Hit
TGGTATTTTCAATAGCCACTAACCTTGCCTGGTACAGTATGGGGGTTGTA6250760.6981099836721318No Hit
GTTTCAGTGAACAAGTTTCAGCGGTTCAACTTTATAATAATTATAAATAA5885410.6573062282032587No Hit
TTTCCATGTAGAAAGGCAGGAATGTCTCCTAAGCTTTCCTGGACAGCAGA5710930.6378196009849503No Hit
TGACCCTTCAAGCTCTGTCCTTATCCGTTACCTTAAAGCAGTCATTCCTT5690170.6355010408001034No Hit
CGCCATGATGTGTAACGAATTCTTTGAAGGCTTCCCAGATAAGCAGCCCA4871650.5440854395235685No Hit
GATGATGATGAAAGTCTTCTTGGACACTTAATGATTGTTGGCAAGAAATG3889390.43438269736712865No Hit
TTCCCTCTCCTGTCCTTGTGTTGAAGGCAGTAAACTAAGGGTGTCAAGCC3776930.42182271285904194No Hit
TGGGATCTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACAT3501010.39100685899570137No Hit
TCCGTCTTATTTCATTTCTGTAACAGTTGATATCTGGCTGTCCTTTTTAT3307230.3693647302567983No Hit
CTTCAGGCTCTTGTTCCGGATGTGGGTCCCTCTCTCTGCCGCTCCTGGCA3238900.3617333614017604No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCACCTGAA3191240.35641050117007433No Hit
GCTGGCCATTATTAAGTCCCTGTAACTCAAATGTCAACCCCACCGAGGCA3172120.3542751027724697No Hit
AAGGCACAGGGAGAAGGGATAACCCTACACCCAGACCCCAGGCTGGACAT3058890.3416291215715925No Hit
AGCCCACTGAACCTCTGCCAATTAAGATGGTGTTGGGTTAGGTTACATCT2891120.32289189410474467No Hit
CCTGTGCTGGCGCTGGAAAATTATTTGTGCTCAGCTGACATTTAACACTC2571660.28721331815815593No Hit
GACTGTCTAAAGGATGCCTGGATTCCTTGTTATCTCAGGACTCTAAATAC2513290.2806943221085647No Hit
ATCTCTGTGACTTCCTGGAGACTCACTTCCTAGATGAGGAAGTGAAGCTT2396160.2676127732429041No Hit
GGTACGGGCCAGGAAGAATATGTGGCAGAGCTCCTGGAAATGATGCAGAT2355790.2631040894923132No Hit
AGAAACAGACTGCCTGAATTGAGAATTTTGATTTCTTAAAGTGTGTTTCT2338720.26119764332876133No Hit
CTCTGAACCCCAATCCTCAAAATGAAGATACTGACACCACCTTTGCCCTC2314870.2585339795325861No Hit
GGTAGGACTGTAGAAAAGGGAACTGAACATTCCAGAGCGTGTAGTGAATC2304200.25734231107534544No Hit
TCGCTTAAGGAATCTGCCCCACAGCTTCCCCCATAGAAGGATTTCATGAG2289750.2557284770353147No Hit
CTCTTCTACAGTACCACCGTCACCTTGTTCAAGGTGAAATGATCCCAACA2169830.24233532976330904No Hit
AACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTGTGACTTC2135540.2385056848337137No Hit
GGCAGACCTGTTATCCTAAACTAGGGTTTTTAATGACCACAACAAGCAAG2032730.22702345108592437No Hit
TTTGCTGTTTCCTTTTATGAGACCCATTCCTATTTCTTATAGTCAATGTT2020130.22561623247662424No Hit
ACAGCATGCCTTCAAATCAATCTGTAAAACTAAGAAACTTAAATTTTAGT2019330.2255268852633353No Hit
AAAACTACTAGGATCACGCGGCATGTATTGAGCATATAGGTTGCTGTAGA2014860.22502765770908364No Hit
GATCTCCTTCGCTGACTACAACCTGCTGGACTTGCTGCTGATCCATGAGG2011710.22467585305675858No Hit
GCCTATCACCTCTTATGCACACCTCCCACCCCCTGTATTCCCACCCCTGG2005370.22396777639144405No Hit
AGCAGGCTGAAGAGGAACGATATTTCCGAGCACAGAGTAGAGAACAACTG1868350.20866483243538828No Hit
GCAACTGCAGAAAATGTCCTCCCTTGTGGCTTCCTCAGTTCCTGCCCTTG1804510.20153492481493426No Hit
TAATCTGATTGGTGGCCTAGCTATGGCTTGCCATGACTCCTTCCTCAAGG1795320.20050854870227808No Hit
GGCCATGATTATCTTAAAGGCATTATTCTCCAGCCTTAAGATCTTAGGAC1793960.20035665843968695No Hit
CCTTCGATCTCCTGACGAGTTTATTGTTGGCCAAAACCAGGCTTTGATTG1784300.19927779083922353No Hit
GACTGTTCACAGACCTCAGTGATGTGAGCACTGTTGCTCAGGAGTGACAA1765380.1971647292449411No Hit
CGCGGCATGTATTGAGCATATAGGTTGCTGTAGATGAATGTTCTTAGCTG1682920.18795526523518805No Hit
CTGAATGTAACCTGCTAGCTCTCCGAAGGCCCTGCGGGCCTGGCTTGTGT1631060.1821633321337353No Hit
GCAATAAGAAATGAACAAAAGATTGGAGCTGAAGACCTAAAGTGCTTGCT1616860.18057741909785738No Hit
GTTGTTTTAGGGGCTAAAGGGGAGAAACTGAAAGTGTTTTACTCTTTTTC1579180.17636916535195035No Hit
GACAGTGATTCCTGCAACTTGACCTTCAGGCTGGGAGAGGTGGAGAGCCA1430210.15973159739739162No Hit
ACAATCCTTGACCTCAGTGAAAGCAGTCATCTTCAGCATTTTCCAGCCCT1416310.15817918956649704No Hit
ATGGCCACAGTAGTCTTGATGACCAAAGTCCTTTTTTTCCATCTTTAGAA1345710.15029429799375188No Hit
GTTTTAGGGGCTAAAGGGGAGAAACTGAAAGTGTTTTACTCTTTTTCTAA1331040.14865589347006675No Hit
ATTTGCAATTTTTGACCCTGACCACTAACTAGTGATTCTTCTCCAAAATT1326960.14820022268229335No Hit
CCCGTCCTGGCTATGAAACCTTGTGAGCAGAAGGCAAGAGCGGCAAGATG1326020.1480952397066789No Hit
CTACAAATGAGCTCTTGCAACAGAACTCACAGCCTTTTTACTTTTTTCCC1318290.14723192225827494No Hit
TGGAGATGACCACGCCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGC1285880.14361224327990849No Hit
ACAATCCTTGACCTCAGTGAAAGCAGTCATCTTCAGCGTTTTCCAGCCCT1233520.1377644681701502No Hit
GAGCAGTGAATCAAGGCAGACTTATGAAATCTGTATTATATTTGTAACAG1208030.13491764258673272No Hit
GTGAGGAAGAAGGAGAGGAATACTAAAGTTAAAACGTCACAAAGGTGCTG1152140.12867562289833714No Hit
TGGGCATTTGCTACATGATGAGTGCTGCCAGATTGTGGCAGGTAAAGAGA1127400.12591256032737802No Hit
AAAACTACTAGGATCACGCGGCATGTATTGAGGTGTGGCATGCAGCATTT1126480.1258098110320958No Hit
ACCTAAATTAAGACTCTGACTTGGATTGTGAATTATAATGATATGCCCCT1097190.12253858618555605No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCGTCTGAA1083840.12104760456379758No Hit
AGAAACAGACTGCCTGAATTGAGAATTTTGATTTTTTAAAGTGTGTTTCT1081160.12074829139927977No Hit
TCAACGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTAC1048650.11711744401925223No Hit
CTCTCGATATTCCTGTGCAGAAACCTGGACCACGTCTACAACCGGCTCGC1043250.11651435032955218No Hit
ATATATTGGCAGCAACTCCGAGAGAAATGCTTGTTATTTTCTCACACTGT1006680.11243006584208347No Hit
TCAACGAGATCGAGACCATCCTGGCCAACATGGTGAAACCCTGTCTGTAC994030.11101726303195279No Hit
GCAATATGTGATGTGGCAAATCTCTATTAGGAAATATTCTGTAATCTTCA991300.11071236566660442No Hit
CCTGATGTGCATATCCGTACTTCCATGAGGTGTTTTCTGTGTGCAGAACA980150.10946708888139042No Hit
CCTGAAGAGACAGCAGAAGACACAACAGAAGACACAGAGCAAGACGAAGA966560.10794930309564528No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCATCTGAA957600.10694861430680963No Hit
TTGTTGAGGTGGTCTGAATGTTCTGACATTAACAGTTTTCCATGAAAACG950740.10618246195285734No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)