FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005475360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142407305
Sequences flagged as poor quality0
Sequence length93
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTAATACCTGCCACCCCACTCTTAATCAGTGGTGGAAGAACGGTCTC1460614110.256595334066605No Hit
CTCTTCAATCTCTTGCACTCAAAGCTTGTTAAGATAGTTAAGCGTGCATA68589694.816444633932227No Hit
CTCATTAAAGCAGCCGGTGTAAATGTTGAGCCTTTTTGGCCTGGCTTGTT58409674.10159226031277No Hit
TTGAAGACGTCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCTGCT45013203.1608771755072533No Hit
GACTGTTCACAGACCTCAGTGATGTGAGCACTGTTGCTCAGGAGTGACAA40173022.8209943303119176No Hit
TGGATCAAGTTTAAATGACTGTGCTGCCCCTTTCACATCAAAGAACTACT32918412.3115675140400977No Hit
ATCTCTGTGACTTCCTGGAGACTCACTTCCTAGATGAGGAAGTGAAGCTT27577381.9365144224869644No Hit
AAGGCACAGGGAGAAGGGATAACCCTACACCCAGACCCCAGGCTGGACAT26769631.879793315378028No Hit
ATGTCCACCATGACCCTCTTCCCACGCTGACCTGTGCTCCCTCCCCAATC25513021.7915527577746098No Hit
AGCCCACTGAACCTCTGCCAATTAAGATGGTGTTGGGTTAGGTTACATCT25343471.7796467674182865No Hit
AGACGGTTGAAACTAGAGATGGACAGGTTATCAACGAAACTTCTCAGCAT25250871.7731442920010319No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCACCTGAA22320831.5673936108825317No Hit
CTCTAGAGGCTGGCAAGAACCAGTTGTTTTGTCTTGCGGGTCTGTCAGGG21855631.5347267473392605No Hit
ACAATCCTTGACCTCAGTGAAAGCAGTCATCTTCAGCGTTTTCCAGCCCT19820191.3917958773252537No Hit
AACTGCACAAACTGGCCACTGACAAAAATGACCCCCATTTGTGTGACTTC18808331.320741938062798No Hit
TGGGATCTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACAT18079401.269555659381378No Hit
CAAGCTCATTTCCTGGTATGACAACGAATTTGGCTACAGCAACAGGGTGG16866691.1843978088062266No Hit
CAACAGAAAAACAATCTTATTCCGAGCATTCCAGTAACTTTTTTGTGTAT15366351.079042258401No Hit
CTTTGTGACTTCAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCATCTGAA14089900.9894085138399326No Hit
TGACCCTTCAAGCTCTGTCCTTATCCATTACCTCAAAGCAGTCATTCCTT13593510.954551453663139No Hit
CGCCATGATGTGTAACGAATTCTTTGAAGGCTTCCCAGATAAGCAGCCCA11897660.8354669727090194No Hit
GCAACTGCAGAAAATGTCCTCCCTTGTGGCTTCCTCAGCTCCTGTTCTTG11090780.7788069579717136No Hit
GTGCTCACCACACATCTGGGGCAGCCTTCCCTGGCAGGGGCCCTCGTGGC10110250.7099530462991347No Hit
GATCTCCTTCGCTGACTACAACCTGCTGGACTTGCTGCTGATCCATGAGG10025700.7040158508722569No Hit
GTTTCAGTGAACAAGTTTCAGCGGTTCAACTTTATAATAATTATAAATAA9910470.6959242715814332No Hit
GTGAGGAAGAAGGAGAGGAATACTAATTATCCATTCCTTTTGGCCCTGCA9299160.6529974006600293No Hit
GATGATGATGAAAGTCTTCTTGGACACTTAATGATTGTTGGCAAGAAATG9238370.6487286589687236No Hit
GCCAATGAATGTACTCTGTGCATGAAAATCAGGGAAGGTGGTCATAATAT8391100.5892324133231789No Hit
AGAAACAGACTGCCTGAATTGAGAATTTTGATTTCTTAAAGTGTGTTTCT8153050.5725162764648907No Hit
TACCCCCGTCCTCTTCGAGACCCCAGTCGTGATGTGGAGGAAGAGCCACC7741580.5436223935281971No Hit
TTCCCTCTCCTGTCCTTGTGTTGAAGGCAGTAAACTAAGGGTGTCAAGCC7600540.5337184072123267No Hit
TTTCCATGTAGAAAGGCAGGAATGTCTCCTAAGCTTTCCTGGACAGCAGA7474710.5248824840832428No Hit
CTTCAGGCTCTTGTTCCGGATGTGGGTCCCTCTCTCTGCCGCTCCTGGCA6900350.48455028342822726No Hit
GGCAGACCTGTTATCCTAAACTAGGGTTTTTAATGACCACAACAAGCAAG6650250.46698798211229403No Hit
TCCGTCTTATTTCATTTCTGTAACAGTTGATATCTGGCTGTCCTTTTTAT6590160.46276839520276014No Hit
TGGTATTTTCAATAGCCACTAACCTTGCCTGGTACAGTATGGGGGTTGTA5971970.4193584029976552No Hit
GCAATAAGAAATGAACAAAAGATTGGAGCTGAAGACCTAAAGTGCTTGCT5885820.41330885378387017No Hit
AGAAACAGACTGCCTGAATTGAGAATTTTGATTTTTTAAAGTGTGTTTCT5618110.39450995860078947No Hit
AGAGGCCGTATTACAAATGAGCCCGAATCATGACAGTCAGCAACATGATA5461840.3835365046757959No Hit
TCAACGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCTCTAC5218780.3664685600222545No Hit
AGCGAGAGAAAAGGAAAAGCCAACAGGCCCCCCAGCCAAGAAAGCTATCT4824800.33880284441869046No Hit
CCTTCGATCTCCTGACGAGTTTATTGTTGGCCAAAACCAGGCTTTGATTG4666590.32769316152707195No Hit
CACCAGCCTGGATAGTCCTGTCACCGTGGAGTGGAAGGCACAGTCTGATT4518590.31730043623815507No Hit
CTGAATGTAACCTGCTAGCTCTCCGAAGGCCCTGCGGGCCTGGCTTGTGT4004960.2812327640074363No Hit
GACAGTGATTCCTGCAACTTGACCTTCAGGCTGGGAGAGGTGGAGAGCCA3847700.2701897911767939No Hit
TCCACCTGGATGAGAAGTCTTTCCGTTGGTTGCACAACGAGGACCAGATG3811690.26766112876021353No Hit
GGATTACTTTTGATCCTGGGGGACCAATGAGGTGAGGGGGGTTCTCCTTT3662790.25720520446616135No Hit
TAGGTGTCTGCCTTCACAAATGTCATTGTCTACTCCTAGAAGAACCAAAT3586220.2518283735514832No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3533860.24815159587494476No Hit
GAGGAATTATGAACTGGGGTAAACCCATTTTGAATATTAGCATTGCCAAT3450580.24230358126642448No Hit
GAATTGGGAGTGAGATGCCTGCATGCTGGGTTCTGCACAGCTGGCCTCCC3228650.22671940881122638No Hit
ACCTAAATTAAGACTCTGACTTGGATTGTGAATTATAATGATATGCCCCT3219310.22606354358015554No Hit
GACTGTCTAAAGGATGCCTGGATTCCTTGTTATCTCAGGACTCTAAATAC3208470.22530234667385915No Hit
TAGAGACCGAGTGTCCTCAGTATATCAGGAAAAAGGGTGCAGACGTCTGG3202140.22485784700440756No Hit
CGCGGCATGTATTGAGCATATAGGTTGCTGTAGATGAATGTTCTTAGCTG3116080.218814617691136No Hit
TTGTTGAGGTGGTCTGAATGTTCTGACATTAACAGTTTTCCATGAAAACG3040630.2135164344272929No Hit
TCCCCGACTCCTGCTGCATTAATGTTACTGTGGGCTGTGGGATTAATTTC2908760.2042563757526343No Hit
GACAAAACGACACCAGCCAAACCAGCAGCCCCTCAGCATCCAGCAGCATG2891920.20307385214543594No Hit
TCGCTTAAGGAATCTGCCCCACAGCTTCCCCCATAGAAGGATTTCATGAG2854300.20043213373078017No Hit
GTGAGGAAGAAGGAGAGGAATACTAAAGTTAAAACGTCACAAAGGTGCTG2836830.19920537081998707No Hit
CTGCACCACACATTCAAAAGGATCGTTTTGTTTTGTTTTTAAAGAAAGGT2782490.195389555332151No Hit
ATGCTGATGGCGAGGCTAACCTGGGCCTCCCACGAGAAGATGCACGAGGG2722590.19118331043481232No Hit
ATTTGCAATTTTTGACCCTGACCACTAACTAGTGATTCTTCTCCAAAATT2714380.19060679506574468No Hit
GGTAGGACTGTAGAAAAGGGAACTGAACATTCCAGAGCGTGTAGTGAATC2690010.18889550644891426No Hit
GAAATCTAGCTGCTGAATACGCTGAAGTAAATCCTTGTTCACTGAAGTCT2633130.18490132932436296No Hit
CTCTCGATATTCCTGTGCAGAAACCTGGACCACGTCTACAACCGGCTCGC2590080.18187831024539083No Hit
GGATGATATACACAGTGGCAAAACAAAAGTACATTGCTTAAAATATATAG2564340.18007081869852112No Hit
TTTGCTGTTTCCTTTTATGAGACCCATTCCTATTTCTTATAGTCAATGTT2491460.17495310370489772No Hit
CCTGAAGAGACAGCAGAAGACACAACAGAAGACACAGAGCAAGACGAAGA2459130.17268285499820393No Hit
TGGAGACGCATTTCGGTTGAGAATGGAAACCACAACTTTTAACCAAACAA2419280.16988454349304624No Hit
TCAACGAGATCGAGACCATCCTGGCCAACATGGTGAAACCCTGTCTGTAC2344000.16459829781906202No Hit
AAAACTACTAGGATCACGCGGCATGTATTGAGCATATAGGTTGCTGTAGA2273570.15965262456164028No Hit
AGCATGAGAAACCACCACAGTAGCTTTAGAATAGATGCGGTCATATTCTT2149970.15097329452305835No Hit
TCTGGTAGTGCTTAACTTTGGGGATGTGGGCCTCTCGGCTGGACTGCAGG2137930.1501278322765816No Hit
GGAGTGCTGCTGTAGATGACAACCTCCATTCTATTTTAGAATAAATTCCC2121850.14899867671816414No Hit
AGCAGGCTGAAGAGGAACGATATTTCCGAGCACAGAGTAGAGAACAACTG2111580.14827750584845348No Hit
TACCCCCGTCCTCTTCGAGACCCCAGTCGTGATGTGGAGGAAGAGCCCCC2108870.1480872066218794No Hit
TTCTACACAGAAGATTCACCTGGGCTCAAAGTTGGCCCTGTTCCAGTATT2001440.14054335204222845No Hit
CTACAAATGAGCTCTTGCAACAGAACTCACAGCCTTTTTACTTTTTTCCC1995020.14009253247226328No Hit
CCTTCTTGGGCCTGCCTCTTAAAGGCCTGAGCTAGCTGGAGAAGAGGGGA1956300.13737357082910878No Hit
TATTGCAGACTTGGAAGGAAGCATAAGCTTCCCATTTCACAAAGGGGAAA1955490.13731669172448702No Hit
CCGCTGGAAAACACTGTCACTTTCAGAGCTCGGTGGCTCACAGCGTGTCC1821610.12791548860502627No Hit
GGCTCTTTCCCTCATGTATACTTCAAGTAAGATCAAGAATCTTTTGTGAA1759280.12353860639382228No Hit
AGGAACTTCAGAAACCCCTTGGCCAAGTAAGCTGTGGGCAGGCAAGCCCT1725640.1211763680240982No Hit
TAATCTGATTGGTGGCCTAGCTATGGCTTGCCATGACTCCTTCCTCAAGG1671950.11740619626219315No Hit
CGTCTTTTACCTGTCACCGAAGCCAGGAAGCCCCGTTTGTAAGCGTGTGT1549650.10881815367547332No Hit
TCAGACTAGTGACAAGCTCCTGGTCTTGAGATGTCTTCTCGTTAAGGAGA1520030.10673820419535361No Hit
CCTAACAGGCTGTAGATTACCAAAACAAGATTAATTTCATCACTAAAAGA1460200.10253687477619214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)