FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005475453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36316227
Sequences flagged as poor quality0
Sequence length37-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG1296790.3570827993778098TruSeq Adapter, Index 1 (97% over 36bp)
GCCCGCTCTCCCAGTCATCACAGTCTGGTTTCTTGATATCCTGAAGGAAGA911970.2511191484732156No Hit
GTCATTTTTGTCAGTGGCCAGTTTGTGCAGTTCCAGTAGTGACTGATTCA846600.23311893055410188No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA803850.22134733324582426No Hit
GTCCGGTCTTCTCCTTGGGGGTCACCCGCACTCGATATCCAGTGAGCTGAA771600.2124670054518604No Hit
CCCGGGGCCTGTGGAAGACTTCACAGCCTTGAGTTGGATGCCAGGCTCGG671580.18492559813551115No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTT656850.18086956004543092No Hit
GCGGGGGCTTTGTATGATTATGGGCGTTGATTAGTAGTAGTTACTGGTTGA515690.14199988341299882No Hit
GGGAGGGCGATGAGGACTAGGATGATGGCGGGTAGGATAGTTCAGACGGTT510520.14057627737595096No Hit
GGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGA468140.12890656289817773No Hit
GTCGGGTGTGTTATTATTCTGAATTTTGGGGGAGGTTATATGGGTTTAATA450340.1240051726739124No Hit
GTCGGAGGAAAAGGTTGGGGAACAGCTAAATAGGTTGTTGTTGATTTGGTT446680.12299735872892302No Hit
GGGGAGGGAGGTGTAGGGGAAGGAGATATGGAGAGGGAGGCAGAGCTACAG422790.11641903218635571No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)