FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005475457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34366830
Sequences flagged as poor quality0
Sequence length39-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG3704471.0779201922318702TruSeq Adapter, Index 1 (97% over 36bp)
GTCCGGTCTTCTCCTTGGGGGTCACCCGCACTCGATATCCAGTGAGCTGAA2575090.7492951779375636No Hit
GGGGAAAGTTGGTTGGGTGGGAGGGAGCCAGGTTGGGATGGAGGGAGTTTA2192030.6378330500660084No Hit
GTGGGAGGGAGCCAGGTTGGGATGGAGGGAGTTTACAGGAAGCAGACAGGG1246500.3627043867589766No Hit
GGGAAAGTTGGTTGGGTGGGAGGGAGCCAGGTTGGGATGGAGGGAGTTTA884080.25724804993652306No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA788620.22947126633442771No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTT746560.21723272120239195No Hit
GCGGGGGAAGGAGTTAATGAAACTGTATTGCACAATGCTCTGATCAATC617560.1796965271455063No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG577580.16806321677035677No Hit
GGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGA529890.1541864640992492No Hit
GCCCGCTCTCCCAGTCATCACAGTCTGGTTTCTTGATATCCTGAAGGAAGA509780.14833489152185408No Hit
GGGGTGTGGAAGGGTTACCAGTTGGGGAAGCTCGTCTGTCTTTTTCCTTC498050.144921716666914No Hit
GTCATTTTTGTCAGTGGCCAGTTTGTGCAGTTCCAGTAGTGACTGATTCA477430.13892174518278236No Hit
CCGGTCTTCTCCTTGGGGGTCACCCGCACTCGATATCCAGTGAGCTGAACA449960.13092857269640523No Hit
GGCGGGGTTCTTGCGGCTGCCCTCTGGGCTCCGGATGTTCTCGATCTG440360.12813518151077652No Hit
GGAAAGTTGGTTGGGTGGGAGGGAGCCAGGTTGGGATGGAGGGAGTTTACA435680.12677340330778253No Hit
CCCGGATACAGGTTTCGCCAGTAGAGAAATCACAGTATACTTTGATAGCAT425810.12390144799505802No Hit
GGGAGGGAGCCAGGTTGGGATGGAGGGAGTTTACAGGAAGCAGACAGGGC421020.12250766218472871No Hit
GGGGAGCGGGGGAAGGAGTTAATGAAACTGTATTGCACAATGCTCTGATCA401790.11691215046601622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG395350.064.1867445
CTCGTAT409050.046.0352944
CGATAGA399150.042.94389736
GATCGGA410500.042.1006241
ACACGTC416250.041.67777613
TCTCGTA418800.041.6613343
ACGTCTG414900.041.65839815
GTCCGGT302350.041.4315571
CACACGT427250.040.58371712
ATAGATC418250.040.4950638
TCGGAAG432750.040.130083
ATCGGAA435300.039.8901752
GATAGAT435150.039.36658537
CACGTCT441300.039.3173114
AACGGAT23600.038.91032445
AGCGATA444350.038.80822434
GCGATAG444550.038.78130735
GCACACG447850.038.70690511
CACAGCG448500.038.44304331
ATCTCGT444300.038.21862442
Produced by FastQC (version 0.11.5)