FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005475477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16045747
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG1323540.8248540875036856No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGT289840.1806335348550616No Hit
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCGCGGCCCCG258380.16102709334753937No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG205130.1278407293845528No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA196000.12215074810789427No Hit
GGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGC184940.11525795589323452No Hit
GCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCGC182550.11376846462804133No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG174330.10864561182474086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGC498650.038.1670847
CAGTCCG492300.037.389836
GTCCGCC527200.036.1625828
TCCGCCC537200.036.0502749
GTTCCGT27000.033.860041
TAGTCCG19650.032.7633136
GTCCGTT20550.030.1965272
GACAGTC495200.030.0644514
ACAGTCC501700.029.7204765
TCCGCTA14750.028.7541121
TCGGCCC248250.028.6404278
CCGTGGG566300.028.5445894
GGACAGT525100.028.2286453
GGGGACA584350.025.4756951
GGGACAG582650.025.4719262
GTGCCGT49100.024.7621121
CCCGTCG391350.023.8624944-45
CCGTTAT10250.023.8412064
TTCCGAC180250.023.4376588
TCGCCGG397300.023.39789248-49
Produced by FastQC (version 0.11.5)