FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005475478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16045747
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT519350.32366832158079023No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT258850.1613200058557573No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCC178220.111069930243821No Hit
CCGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT172620.10757990886930972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT236400.059.524059
CATAGCG26150.048.70621594
TACGGCC40050.047.762627
TCTACGG40750.047.520035
GTCTACG41000.046.9999774
CGGCAAT26650.044.9895551
GCGCTAT316250.044.13800494
CTTAGCG79800.043.1118169
CGGGTAG42500.043.094121
CGGCATT45950.043.0431141
CGGTTCA33500.039.0038155
CCGGTAG22800.038.5082051
CGGCAAA52450.038.1588751
GCCGGTT35050.038.0831953
CGGGTAT24350.035.8631131
CTACGGC54250.035.4340826
GGCGCGG404050.035.361068
CGGGAAT43400.034.913271
CGGTAAG15750.034.4661251
CGGCAAC50200.034.321351
Produced by FastQC (version 0.11.5)