FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005475481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22074173
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG807430.3657804077190117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCGC346150.033.627817
CAGTCCG341200.033.1377876
GTCCGCC377900.030.8771158
TCCGCCC402650.029.4935659
CGTCCGT32750.027.6239191
GTTCCGT28650.026.314231
GTCCGTT26350.024.9766332
CGCAAAC171700.024.74767594
ACAGTCC380750.024.0675355
GACAGTC377550.024.0480794
TCGTACG44900.023.86591312-13
CCCGTTA25300.023.776723
GTCCGTC53350.023.7030542
CCGTTAT18750.023.5599484
CCGCTAA55400.022.9108122
GTGCCGT56550.022.8303871
CCGTTAA22700.022.7727054
GGACAGT403050.022.597343
CTCCGAC191100.022.4276549
GTCGCGT33000.021.6460869
Produced by FastQC (version 0.11.5)