FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005475482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22074173
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT414040.18756761578338632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT210650.051.3818979
TGACACG60600.051.033066
CGGCAAT38350.049.1080551
GCGCTAT241500.044.6054594
CGGGTAT38400.044.373241
CGGGTAG62300.043.3364371
CGGCATT67300.043.2026981
GACACGC71700.043.132257
CGGCAAA82650.042.203341
ACACGCT73700.042.1513678
CCGGTAG31500.039.708231
CGGGTAA44350.037.994421
CGGTAAG27350.036.93191
CGGGAAT63250.036.1931531
CGGCATA43200.035.9465641
GAACGGA61500.035.0021363
CGGCAAC67150.034.7940031
CATAGCG28800.034.5966394
CGGCAAG106950.034.2472841
GCGCATA14500.034.179561
Produced by FastQC (version 0.11.5)