FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005475486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32520978
Sequences flagged as poor quality0
Sequence length100
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT879110.2703208987134397No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT424580.1305557292895681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT419850.056.0706069
CATAGCG46950.047.94989494
GCGCTAT540300.043.17149494
TCTACGG81950.040.892315
GTCTACG86000.039.0750924
CGGCATT91150.038.6891751
GCCGGTT66200.037.4863973
TACGGCC90300.037.474047
CTTAGCG128700.037.4303369
CGGCAAT58450.037.3957251
CGGGTAT46000.037.3567471
CGGGTAG85400.037.2586861
CCGGTAG41300.036.4641531
CGGTTCA67450.036.443565
CGGCAAA114700.034.0794371
CCGGTAA27600.033.6963351
CGGTAAG24450.033.403561
CGGCAAC117250.032.170621
CGGGGAT167750.031.2944831
TGACACG61850.031.2313356
Produced by FastQC (version 0.11.5)