FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005475491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25071461
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT5647252.2524614740241904No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG4111431.6398844885824562No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG3681001.4682032291616351No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC2502950.9983263440451277No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT2298010.9165839996320917No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT2006180.8001847199889947No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC1578970.6297877893912923No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG1339020.5340813604759611No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC1331880.531233500911654No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG1186590.4732831485169532No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA1109550.4425549831340104No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA1083930.4323361929326735No Hit
CCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCACGCGCCCTCCCGCG1070120.42682793794904894No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG797500.31809075665753983No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA670600.26747543751040276No Hit
GCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCG664840.2651780045845753No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC642500.2562674747993346No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC609410.24306920127231516No Hit
AGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTG594180.23699456525489282No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT591840.2360612331287754No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG573400.22870625688706375No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA568550.22677178645472634No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT566230.22584643152626804No Hit
GCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCAC537100.214227643135755No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC495770.19774276417317682No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG488950.1950225397714158No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA471550.18808237780797857No Hit
GCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAG455240.18157697311696355No Hit
GGCCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGAT432490.17250291077971083No Hit
GCACGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCC417700.1666037731107892No Hit
TCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTG406260.1620408160497707No Hit
ACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGG403560.16096389436578906No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG381480.15215706815011698No Hit
TGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTG380230.15165849329642178No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT366310.14610636372567198No Hit
AGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTA364740.14548015370943082No Hit
GGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTC346660.13826876702558338No Hit
AAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC329330.1313565252539531No Hit
CCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCA324120.12927846526375147No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA321330.1281656461903038No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC313520.12505055050441616No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG310340.12378217607661557No Hit
CTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCT302770.1207628067626374No Hit
CCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAG300700.11993716680491816No Hit
CCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTA288470.11505911043636428No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC283150.11293717585903748No Hit
GCCCTTCACAAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTC282410.11264201954564992No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG279510.11148532588507705No Hit
GCCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCG274260.1093913114995572No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT273630.10914002977329483No Hit
ACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCA273570.10911609818031745No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG265220.10578561815763349No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG261700.1043816313696278No Hit
GTACCGCCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCG261520.1043098365906957No Hit
AAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCAT257880.10285798661673526No Hit
TTCCCCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGA251470.10030129476698625No Hit
CTCTCGGGGCGAACCCATTCCAGGGCGCCCTGCCCTTCACAAAGAAAAGA251330.1002454543833724No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)