FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005475496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20518720
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTG75600.044.309665
GGTGTGC239700.041.3290826
GTGTGCT324850.040.675767
GTGCTCT329950.040.219599
TGTGCTC333550.039.230758
CCGGTGT51350.032.247834
GGTCCGT22750.031.9084341
CCGATCT211050.031.50902216-17
TTCCGAT217800.031.43690914-15
TCCGATC207400.029.8100616-17
CCGATCG29900.029.16767386-87
GTCCCGT20250.028.3501761
TCTTCCG253550.028.11714412-13
CTTCCGA227750.028.1050914-15
ATAGCGC30250.026.7891794-95
CGGGTGT50650.025.7613584
GGTCCGA11650.025.249991
CCGCGTA6800.025.1181052
CGATCTA52000.025.09163918-19
CGATCGG32550.025.04090786-87
Produced by FastQC (version 0.11.5)