FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005475508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22590139
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT827130.3661464854200322No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT429890.19029984720324208No Hit
CCGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT296220.1311280112087845No Hit
GCGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT226930.1004553358436617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT363750.064.744239
CATAGCG40900.056.4218794
GCGCTAT450450.051.75166794
GCCGGTT54900.044.089263
CGGTTCA55950.042.9265025
CGGCAAT38850.042.8933141
CCGGTAG30100.042.6587681
GGCGCGG597900.039.805668
CGGTAAG18800.039.6739231
CGGCATT59950.039.13571
CTTAGCG94900.037.3904279
CGGCAAA72900.037.2993161
CGGGTAG55100.036.3262181
CCGGTAT19000.036.274811
CGGGTAT30450.035.9671971
CGGGTTA29450.035.906131
CCGGTAA19700.035.7047461
CCGGGCG678850.035.0887155
CCGGTTA19750.034.180221
TGGCGCG367850.033.4226114-15
Produced by FastQC (version 0.11.5)