FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005475510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33903214
Sequences flagged as poor quality0
Sequence length36-51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG535970.15808825676527305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCGT52500.017.1638725
AGTCGTC53600.016.7294736
GCGGGGT48650.016.428339
AGAGTCG60400.014.8081334
TCGTCGG62750.014.1150718
GTACCGT50000.013.54707545
GTCGTCG65600.013.5010637
CGGGGTG69350.012.9404140
CGAGAGT73750.012.7922912
TAGCGTA5400.012.779325537
CTTGACG24350.012.065531
CGTCGGG73700.012.0181169
GGTGATA61000.011.9187143
GGGTTAT37150.011.33595642
GCTCGTT55450.011.16765743
GGGAAAA265200.011.03692222
GGGTGAT81800.010.81690342
CAGTAAG70350.010.79541245
GCGTCGT16200.010.71578712
GGGGGGT268300.010.69273914
Produced by FastQC (version 0.11.5)