FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005475518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24449207
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT461580.1887913992466095No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGAAC234650.038.1513472-73
GTACGGT26000.037.0236552
CGACCTA10000.036.0408171
GTAGCAA373000.035.9884879
TAGTAGC370700.035.7683037
GCAGTTG263550.034.42041868-69
AGTAGCA395350.033.9999358
CGAGTGC67200.032.2052768-69
GCGAGGG123500.032.19137268-69
CAGTTGA294450.031.00216370-71
AGTACGA91250.030.70199
CAGTACG91450.030.684468
AGTTGAA292150.030.4140970-71
GTCCCGT25050.030.232071
CCCGCGA147100.030.18503464-65
GTAGTAG289750.030.0130796
TCAGTCG287200.029.79871284-85
TGATCGT51350.029.5880725
CGGTGAA177350.029.3797285
ATACCGG20100.029.2835259
Produced by FastQC (version 0.11.5)