FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005475524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17206061
Sequences flagged as poor quality0
Sequence length100
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT495090.2877416277903467No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT259510.1508247587870344No Hit
CCGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT179510.1043295150470523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT239450.058.4508449
CATAGCG26200.049.15146694
GCGCTAT302650.045.71769794
CGGCAAT29850.043.966011
CGGCATT47200.042.50731
CGGGTAT25150.041.107661
CGGGTAG45700.040.9068071
TCTACGG34150.039.7748225
TGACACG37500.039.730046
CCGGTAG23950.038.8308831
CGGCAAA54600.038.3025471
GCCGGTT35750.038.1261633
CCGGTAT16000.036.8813321
CGGTTCA36750.036.5771455
GTCTACG38550.036.3316734
CTTAGCG70450.036.2242779
TACGGCC38800.035.976137
CGGGAAA93000.034.314711
CGGGTAA33700.034.180351
CCGGTAA15500.033.80711
Produced by FastQC (version 0.11.5)