FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005475525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18842684
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG1236460.6562016324213684No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGT300720.15959509802319033No Hit
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCGCGGCCCCG255210.13544248791732644No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG190490.1010949395531974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCCG456900.037.5933766
AGTCCGC467900.037.4226767
TCCGCCC500800.035.2838139
GTCCGCC500250.035.0308278
GTTCCGT28650.034.2006031
GTCCGTT22300.032.0439722
CGCAAAC196450.031.12798594
ACAGTCC469400.030.575325
GACAGTC472550.030.452524
GGACAGT496700.028.9165763
GTCCGTC48450.026.8777922
CGTCCGT31000.026.7452091
TCGGCCC226250.025.5482758
GGGGACA603200.024.4677541
CTCCGAC245500.024.0047959
GGGACAG610500.023.8948152
GCGCTAG26350.023.00788128-29
TCGTACG29150.022.73381412-13
TCCGCTA16050.022.600121
CCGTGGG673600.022.58740694
Produced by FastQC (version 0.11.5)