FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005475526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18842684
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT543190.28827634109875216No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCC445100.23621900149681435No Hit
CGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCC288640.15318412175250617No Hit
GGGGGCCCGCGCCTCCCCCTTCCGAGTCGGGGGAGGATCCCGCCGGGCCG261530.1387965748403996No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT245640.13036359363666025No Hit
GGGGGCCCGCGCCTCCCCCCTTCCGAGTCGGGGGAGGATCCCGCCGGGCC202560.10750060872431974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT251300.057.2658049
CGGCAAT34250.051.2578051
GCGCTAT299300.047.7677994
CGGGTAT27350.045.5537571
CGGGTAG54150.045.319231
GAACGGA59400.044.4676363
CATAGCG22950.044.23374694
CGGCAAA68800.041.431071
CGGCATT51850.041.2313041
CCGGTTG30450.040.6061331
CTTAGCG72400.040.5717859
GGGTCGT46050.040.31552
TACGGCC33050.039.9593737
CGGCACT70550.038.463471
CGGGTAC38050.038.4489671
TGACACG41850.037.958356
CGGCACA66050.037.7972761
TCTACGG34900.037.573355
CCGGTAT16450.037.0084951
CCGGTAG25450.036.1596681
Produced by FastQC (version 0.11.5)