FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005475528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005475528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24254487
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560840.2312314418358962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGA111850.041.245318
CGGACTA14050.039.621455
ATCGAGA58600.038.57076672-73
ATCGCCG29100.036.62858268-69
TCCGTTA30800.036.2955443
CCGGTCG30600.034.36632572-73
GTCCGTT21500.034.165782
TATCGAT49900.034.1089027
AATCGAG68600.033.50312470-71
TCCGACC33500.032.8275079
CGTTATC103550.032.564265
TTATCGG144600.032.0931327
CCGTTAT25700.031.7806824
CCCGTTA24500.031.6607193
CCGTTTA43750.031.6015154
CGTACGA7550.031.4270255
AGACCCG141650.030.95525274-75
ATAGCGC65550.030.7178994-95
CCGATCG70200.030.54420386-87
GTTATCG123900.030.4563266
Produced by FastQC (version 0.11.5)