FastQCFastQC Report
Fri 13 May 2022
EGAF00005478126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005478126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024983071
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCCGTGAAGGTGTAGATCTC106421371.0382744165342415Illumina Single End PCR Primer 1 (96% over 31bp)
TCACCTGTCGGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT60978850.5949254356026334No Hit
TCACCTGTCGGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA44813290.4372100502721376No Hit
TCACCTGTCGGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC13367560.13041737349825946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)