FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005478135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005478135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810723812
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACGCTGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT61838990.7627627199878027No Hit
CCCTAAACGCTTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA47030720.5801077913818572No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCGAGGATCTCGTATG42864930.5287242013313407TruSeq Adapter, Index 10 (97% over 36bp)
CCCTAAACGCTGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC11551980.142489708936784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAACG14530400.0134.576773
AAACGCT15608750.0125.201245
TAAACGC15824900.0123.599044
AACGCTG10620950.0115.156026
AACGCTT6471300.0113.321646
CGCTGGC9073000.0109.323648
CCTAAAC18148450.0108.02682
ACGCTTG7245350.0101.412467
GCTGGCG9882000.0100.481639
CCCTAAA20803850.094.638131
CGCTGTA2495700.092.274448
ACGCTGG10879800.091.450047
ATCGGAA8436100.086.93861
TCGGAAG8727350.083.7734762
CTCGCCC8459900.082.78555145
CGGAAGA8974750.081.4219363
ACGCTGT3224400.071.8410647
CGCTTGA10498700.069.8023768
GACATAA14147950.067.724625145
AGAGCAC13252500.055.395667
Produced by FastQC (version 0.11.5)