FastQCFastQC Report
Fri 13 May 2022
EGAF00005478137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005478137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877558909
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NGCTTAAGAGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT17019370.19393991475049796No Hit
NTCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGGCAATCTCGTATG14406130.16416140104390414TruSeq Adapter, Index 4 (97% over 34bp)
NGCTTAAGAGATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA9662400.11010542883110312No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)