FastQCFastQC Report
Mon 30 May 2022
EGAF00005478150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005478150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009175254
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAATACAGGTGTAGATCTC35793670.3546823989007562Illumina Single End PCR Primer 1 (96% over 31bp)
ACCCTGGCCTGGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT34165760.3385513057774601No Hit
ACCCTGGCCTGTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA27219960.2697248063912594No Hit
ACCCTGGCCTGGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC12309800.12197881340439606No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)