FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005478192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005478192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117909903
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT27035852.2929244543607163Illumina Single End PCR Primer 1 (100% over 50bp)
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA3131760.2656061891595314No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT2908170.2466434053465382No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG2501980.21219422087048959No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT2042870.17325686375978105No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT1598160.1355407781142861No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA1589810.1348326102854991No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1520940.12899170988207836No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG1437240.12189306949052447No Hit
GTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACC1368430.11605725771820878No Hit
TGGGGATCGGGGATTGCAATTATTCCCCATGAACGAGGAATTCCCAGTAA1290850.10947765769937068No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA1262180.10704614013633783No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC1235000.1047409902457472No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1228390.10418039271900682Illumina Single End PCR Primer 1 (100% over 50bp)
CCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTC1187870.1007438705127253No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)