FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005478199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005478199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102731798
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT6314340.614643189638324No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC5758830.560569376971286No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2940390.28622004649427046No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT2042320.19880115404969353No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA1365240.13289361488640547No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC1225650.11930580636776163TruSeq Adapter, Index 10 (100% over 50bp)
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG1166480.11354614858390778No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1140360.11100360571903939No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1133590.11034460820008231No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1099180.10699510973223696No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA1062550.10342951458904671No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)