FastQCFastQC Report
Fri 27 May 2022
EGAF00005478201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005478201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82521002
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC4400860.5333018132765766TruSeq Adapter, Index 9 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1731860.20986899795521147TruSeq Adapter, Index 9 (100% over 50bp)
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1543610.1870566234762879No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1493090.18093454560815925No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1473890.17860786518321725No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1456960.17655626624601578No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1174680.14234921674848303No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1078980.1307521690054127No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1064030.12894050898703338No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1042940.12638479595776117No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)