FastQCFastQC Report
Thu 26 May 2022
EGAF00005478244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005478244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246860425
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT18464350.7479671964430913Illumina Single End PCR Primer 1 (100% over 50bp)
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG15602650.6320433905110551No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG12151540.49224333953083005Illumina Single End PCR Primer 1 (100% over 50bp)
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA4577730.185437985857798No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC3738040.15142321820113533No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC3485440.14119071536071445No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC2709590.10976202443141705No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)