FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005478256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005478256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88015387
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT3862770.43887439817767315No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA2083370.23670520246647325No Hit
CGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG1528680.1736832674495881No Hit
GGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTC1245960.1415616112669027No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA1098580.124816811860408No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG1089530.12378858255772936No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1048590.11913712314870581Illumina Single End PCR Primer 1 (100% over 50bp)
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG1048000.119070089415161No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA994390.11297910898238736No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)