FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005478267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005478267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91944425
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC47544275.170979099602831TruSeq Adapter, Index 7 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC39412394.286544834012503TruSeq Adapter, Index 7 (100% over 50bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1723740.18747629342398953No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1637190.17806299838190298No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAACTCGTATGCC1396960.1519352587174263TruSeq Adapter, Index 7 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAGCTCGTATGCC1138940.12387265459542543TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAGCTCGTATGC1027220.1117218363157962TruSeq Adapter, Index 7 (98% over 50bp)
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT965220.10497863247282258No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)