FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005478273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005478273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134350798
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG35202852.620218898885885TruSeq Adapter, Index 16 (97% over 39bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG3595520.26762178219440125TruSeq Adapter, Index 16 (97% over 39bp)
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC2897830.21569131282718543No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT2397930.1784827508058419TruSeq Adapter, Index 16 (97% over 40bp)
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT2354380.1752412367509719No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2160530.16081259152625205No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1555540.11578196952726698No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1520170.11314930931783523No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)