FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005478289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005478289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148282651
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC15921721.0737412564872475TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC13975480.9424892194569681TruSeq Adapter, Index 10 (100% over 50bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT4644700.31323286768052183No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT3016160.2034061287452974No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2719340.1833889522247616No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2407950.16238919278560782No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1824390.1230346225736145No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1763030.11889657947914622No Hit
NTCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC1733210.11688555527645644TruSeq Adapter, Index 10 (98% over 50bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1561250.1052887839184909TruSeq Adapter, Index 10 (98% over 50bp)
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1518980.10243814699536227No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)