FastQCFastQC Report
Fri 27 May 2022
EGAF00005478307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005478307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103417747
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC2216340.2143094453604757TruSeq Adapter, Index 10 (100% over 50bp)
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1682500.16268967839726775No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1655180.16004796546186603No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1250600.12092702038848323No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1193670.11542216250369484TruSeq Adapter, Index 10 (100% over 50bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1065780.10305581304145023No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1050150.10154446702460072No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)