FastQCFastQC Report
Sat 25 May 2024
EGAF00005478312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005478312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92340372
Total Bases13.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT10923731.1829852710578208Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4400630.47656619793561156Illumina Single End PCR Primer 1 (100% over 50bp)
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG4182310.4529232349204744No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGA1102160.11935841020870047No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)