FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005478327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005478327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98143729
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT9228470.9403015448903516No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC5359740.5461113057972354No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG2552750.2601032206550864TruSeq Adapter, Index 22 (97% over 39bp)
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT1644490.16755935572816885No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT1078530.10989291022353552TruSeq Adapter, Index 22 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)