FastQCFastQC Report
Fri 27 May 2022
EGAF00005478328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005478328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98143729
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG8868290.9036023075911453No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT2596700.2645813468122859Illumina Single End PCR Primer 1 (100% over 50bp)
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC1389370.14156482682658206No Hit
GCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGG1192100.1214647142661555No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1089110.11097092102542792Illumina Single End PCR Primer 1 (100% over 50bp)
AGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGT1089090.1109688831978251No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG1016870.10361028772403788No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)