FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005478345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005478345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85141552
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2683600.3151927510083443TruSeq Adapter, Index 5 (100% over 50bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2223960.2612073597155006No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1144010.1343656502761425No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC902410.10598937637406468TruSeq Adapter, Index 5 (100% over 50bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA899570.10565581421395748No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)