FastQCFastQC Report
Sun 3 Jul 2022
EGAF00005478346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005478346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85141552
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3150870.3700742969778141Illumina Single End PCR Primer 1 (100% over 50bp)
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT2238660.26293389624844987No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA1343280.15777020367211536No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA1114500.13089965754911304No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1104870.12976859994283402Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)