FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12706041
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT3649402.8721771006405534Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT827750.0126.8631442
TGCAATT900300.0125.30963
ACTGCAA850500.0124.091061
CGCAATT111600.0121.1226353
ACCGCAA114200.0113.544071
CCGCAAT114850.0112.588672
GCAATTT1161450.0109.17944
AATTTCC1227550.0106.477636
CAATTTC1206450.0106.234715
TTTCCAC1261050.0103.1538
ATTTCCA1282550.0101.711857
TTCCACA1294200.0100.754719
TCCGATC51750.041.190885
ATGCAAT123850.039.7181432
CGGCAAT28300.039.597932
CCGATCT57900.038.9129946
TTCCGAT54550.038.4097564
ATTGCAA89350.033.245451
GACCGGA36650.032.9588742
ACGGCAA36300.030.6867871
Produced by FastQC (version 0.11.5)