FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3624456
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT44113012.170929927139412Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA95660.2639292627638465No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA81650.22527518612448322No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT68080.1878350847685832No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCATTT62380.1721085867782641No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAGA55400.15285052432696108No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAG52190.1439940228271498No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCTTTT51450.14195233712314345No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGAAA49610.13687571321047903No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAG46830.12920559664677955No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGAGG43520.1200731916734539No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAACA43270.11938343298966796No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAT42480.11720379554890444No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAC42110.11618295269690128No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCCAGG41990.11585186852868404No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAGAA41930.11568632644457541No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAGG39750.10967163072196215No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGCAGA39150.10801620988087592No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGAGA38710.10680223459741268No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAACA38630.10658151181860119No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCCTTT38260.10556066896659803No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGGGG37380.10313271839967157No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGGGA36460.10059440644333936No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAATT36410.10045645470658218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1175000.0143.087131
CTGCAAT1180900.0141.724182
CCGCAAT173200.0140.085742
ACCGCAA178750.0136.238831
CGCAATT192050.0132.728653
TGCAATT1398700.0132.355833
CGGCAAT17850.0128.232392
TATGCAA56600.0117.296811
GCAATTT1989700.0106.6430054
CAATTTC1989250.0106.5758065
AATTTCC1989750.0106.1868066
TTTCCAC1984450.0105.714368
TTCCACA1993300.0105.441359
ATTTCCA1998950.0105.41897
ATTGCAA43150.0103.808221
ACGGCAA22850.0100.879961
GGCAATT24400.096.190483
ATGCAAT141300.090.252722
GCAATTC30850.071.1310354
ACGCAAT14750.069.462832
Produced by FastQC (version 0.11.5)