FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3624456
Sequences flagged as poor quality0
Sequence length6
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCG245090.6762118232363697No Hit
GGGGGT170480.4703602416472982No Hit
GGGGGG165850.4575859108235829No Hit
GGGAGG139510.3849129358998978No Hit
GGGTGG124040.3422306685472247No Hit
TGGGGG121110.3341466967732537No Hit
GGCGGG118210.32614549604133697No Hit
ACGGGG108420.29913454598428013No Hit
GGGCGT104860.28931238232716855No Hit
GGGGGC102680.2832976866045553No Hit
CGGGGT102630.2831597348677981No Hit
GTAGTC101760.2807593746482231No Hit
GGGGCG97070.2678195017403991No Hit
CTAATC93800.2587974581564792No Hit
GGGCAG93570.25816288016739614No Hit
CGGGGG91300.25189987131861996No Hit
AGGGGG86020.2373321679170612No Hit
CTGAGG85950.23713903548560114No Hit
GTAGCC82830.22853084711195282No Hit
GGAGGG81770.2256062702927005No Hit
CGAGGT81500.22486133091421168No Hit
CTTGGT80600.22237819965258235No Hit
GTTGAG78760.21730157573991793No Hit
TCTCGT77660.21426663753125988No Hit
TCTCAA77550.21396314371039407No Hit
GGTGGA76970.2123629035640107No Hit
CAGGCC76970.2123629035640107No Hit
GTCAGG76030.20976941091297563No Hit
TGCGGG75760.20902447153448683No Hit
GGGCGG75480.20825194180864656No Hit
TGGAGG75320.2078104962510236No Hit
GAGGTG74960.20681724374637186No Hit
ATTGGG74720.20615507540993738No Hit
TGTCGG73550.20292700476981923No Hit
AGGGAG72800.20085772871846147No Hit
GCAGGG70230.19376700944914216No Hit
GAGTGC69040.19048375811432117No Hit
TCAGGG68840.18993195116729242No Hit
TGTGCG68610.18929737317820938No Hit
GAGTGT68380.18866279518912632No Hit
TGGTGG67900.18733845851625736No Hit
GGGGAG67080.1850760500334395No Hit
GTAACC66690.18400002648673344No Hit
GAATCA65450.18057882341515527No Hit
GACGGA65330.18024773924693802No Hit
CGAGGG65310.18019255855223515No Hit
GGTGGT64580.17817846319558023No Hit
CCAGTA63080.1740399110928647No Hit
CGCTTC63010.17384677866140463No Hit
GGGACG62850.17340533310378164No Hit
TGCGGT62550.17257762268323854No Hit
GCGAGG62180.17155677983123535No Hit
GTAGGT61990.17103256323155808No Hit
GGGGCT61900.17078425010539514No Hit
CGGTGG61810.1705359369792322No Hit
CAGGGT61560.16984617829544627No Hit
TGTGGG61160.16874256440138877No Hit
TGGGCT60980.16824593814906294No Hit
AAGTAG60760.16763895050733132No Hit
GAGCGT60460.1668112400867882No Hit
CGGGGC60150.16595593931889363No Hit
GGCCGG59850.16512822889835052No Hit
GGGGCA59680.1646591929933761No Hit
GCTGGG59550.1643005184778074No Hit
CCGCTA59320.16366594048872438No Hit
AGGGGC59080.1630037721522899No Hit
GGGGTG58790.1622036520790982No Hit
GCGTCC58350.160989676795635No Hit
CGCTCA58120.16035509880655194No Hit
TTAGCC57260.15798232893432834No Hit
CTGGGG56580.15610618531443063No Hit
ATTTGG56250.1551957038518332No Hit
TCCGCT56220.1551129328097789No Hit
CCCTGG55590.15337474092663836No Hit
GTGGAG55480.15307124710577255No Hit
GTGAAT55480.15307124710577255No Hit
GGGAGA55430.15293329536901537No Hit
CCAGGG55370.15276775328490674No Hit
AGTCGG55370.15276775328490674No Hit
GGGAGT55340.15268498224285243No Hit
GGGGCC54740.15102956140176624No Hit
GCGGGC54710.15094679035971192No Hit
GGGCTG54660.15080883862295474No Hit
TGGGGT54430.15017426063387168No Hit
ACGCTG54200.14953968264478862No Hit
AAGGGG54200.14953968264478862No Hit
TGAACC53870.14862920118219122No Hit
GTGGTG53180.14672546721494203No Hit
CATGCA52820.14573221471029033No Hit
CCGCGG52640.14523558845796447No Hit
CGCCAA52430.14465619116358427No Hit
TAGCTT52130.14382848074304116No Hit
GGCGCC51940.14330426414336386No Hit
CGGCGC51600.142366192333415No Hit
AGGGTG51320.14159366260757478No Hit
TTGCGG51050.14084872322908595No Hit
GGTGCT50690.13985547072443422No Hit
GAAAGG50410.139082940998594No Hit
AATGGG50290.13875185683037675No Hit
ACTGAG50260.13866908578832243No Hit
GCCTGG50100.13822764023069944No Hit
ATGTAG49880.13762065258896783No Hit
AGCGTG49720.13717920703134484No Hit
GATAGT49300.1360204124425845No Hit
AGGCAG49050.13533065375879857No Hit
AGCCCG49020.13524788271674423No Hit
TGTTGG49010.1352202923693928No Hit
AGAATT49000.1351927020220414No Hit
AGGTGC48820.1346960757697155No Hit
GGGGTC48780.13458571438030978No Hit
AGCGCG48710.1343925819488497No Hit
TGAGGT48160.13287511284452067No Hit
ATCACG48140.1328199321498178No Hit
GAACAT48120.13276475145511493No Hit
CCGGTT47980.1323784865921948No Hit
AGAATG47880.13210258311868042No Hit
AGCGGG47650.13146800512959736No Hit
TGGGAC47440.1308886078352172No Hit
CTCCCA47430.13086101748786577No Hit
GCGTGC47310.13052993331964852No Hit
GCTGTT47300.13050234297229707No Hit
ACTGTA47280.1304471622775942No Hit
TTGATC46970.12959186150969967No Hit
CTTTGG46930.1294815001202939No Hit
ACAGCC46620.12862619935239936No Hit
TATGTT46600.1285710186576965No Hit
GCCGGG46590.12854342831034507No Hit
ATAAAG46490.12826752483683068No Hit
ATGGGG46410.1280468020580192No Hit
TTAAGA46260.12763294684774762No Hit
CGTGTG46250.1276053565003962No Hit
TCTGTT46160.12735704337423326No Hit
TGGGTG46010.12694318816396172No Hit
CGCGGG45860.12652933295369015No Hit
GAGGGG45780.12630861017487865No Hit
TGCGAG45540.12564644183844417No Hit
ATGATA45320.12503945419671256No Hit
CAGCAG44860.12377029821854645No Hit
GTGTGC44850.12374270787119501No Hit
GCATAG44650.12319090092416628No Hit
GAGATT44360.12239078085097461No Hit
GTTGCA44360.12239078085097461No Hit
CGGAGG44210.12197692564070305No Hit
GGCGTG44140.121783793209243No Hit
GTCTAT44070.12159066077778292No Hit
GGAGTC44060.12156307043043148No Hit
TCCGGG43970.12131475730426855No Hit
TAACAT43810.12087331174664556No Hit
GCAGTG43680.12051463723107689No Hit
CGGAGC43630.1203766854943197No Hit
CGCGTG43400.11974210750523664No Hit
GTGGGC43260.11935584264231654No Hit
GTGAGA43230.11927307160026221No Hit
AGACTG43160.11907993916880216No Hit
CAGGCG43120.11896957777939642No Hit
TGCCGT43110.11894198743204497No Hit
CACAGT43030.11872126465323349No Hit
ATGTGG42970.11855572256912485No Hit
GCAAGG42870.11827981909561047No Hit
TTCAGT42570.11745210867506736No Hit
GGGTCG42560.11742451832771594No Hit
GTCGCA42530.11734174728566163No Hit
GGAGTG42460.11714861485420157No Hit
AGGTGT42390.1169554824227415No Hit
CTTCGT42220.11648644651776707No Hit
CTTGCC42180.11637608512836133No Hit
TTGGGG41950.11574150713927828No Hit
AAAGTG41930.11568632644457541No Hit
AGGGCA41820.11538283262370959No Hit
AATGTG41800.11532765192900672No Hit
TCCACG41320.11400331525613776No Hit
TCGAAC41270.11386536351938056No Hit
GGTAGT41020.11317560483559465No Hit
GCGGAA40940.11295488205678313No Hit
CATCCT40870.11276174962532308No Hit
GCCAAA40840.11267897858326878No Hit
TATGGA40780.11251343649916015No Hit
TGGAGC40710.11232030406770009No Hit
GGACCG40680.11223753302564576No Hit
GGTGAC40540.11185126816272567No Hit
GTCTGG40170.11083042531072249No Hit
CAGTAA40090.11060970253191099No Hit
CGCGAG40070.11055452183720811No Hit
CAAAAC40060.11052693148985669No Hit
GGGTGT40000.11036138940574806No Hit
TGCTGG39970.11027861836369376No Hit
GCGAGT39920.11014066662693657No Hit
TGGATG39760.10969922106931357No Hit
TGATGG39720.10958885967990782No Hit
AGGGGA39610.10928536585904203No Hit
TAAGGG39610.10928536585904203No Hit
TCACCA39600.10925777551169058No Hit
GTCAAG39560.10914741412228483No Hit
TAAGAG39330.10851283613320178No Hit
GGTGTG39190.10812657127028166No Hit
TTCTGG39150.10801620988087592No Hit
TGCGCC39000.10760235467060436No Hit
GAAAAG38960.10749199328119863No Hit
GTGGGG38920.10738163189179288No Hit
TAGAGG38640.10660910216595262No Hit
GTGAGG38550.1063607890397897No Hit
GGGTAC38490.10619524695568107No Hit
AAATGT38420.10600211452422101No Hit
GAGCTA38350.10580898209276096No Hit
TTGGTC38320.10572621105070663No Hit
GAGACG38310.1056986207033552No Hit
TGAATA38270.10558825931394945No Hit
AGGAGG38080.10506404271427217No Hit
TTGTAG38030.10492609097751497No Hit
GAGGAC37880.10451223576724342No Hit
AGTAAA37870.10448464541989197No Hit
TGAGAC37720.10407079020962043No Hit
TCGGTT37710.10404319986226898No Hit
TCCAAC37510.10349139291524025No Hit
CGCCTG37500.10346380256788881No Hit
CTGCCC37250.1027740438841029No Hit
CGTATG37180.10258091145264282No Hit
CGGGGA37110.10238777902118276No Hit
TTCGGC37060.10224982728442558No Hit
GAGTAT37000.10208428520031695No Hit
ACTACT36930.10189115276885691No Hit
GGGTCT36920.10186356242150546No Hit
CGCAGA36900.1018083817268026No Hit
GCGCGG36890.10178079137945116No Hit
TGGGTC36700.10125657477977384No Hit
TCAGTG36680.10120139408507098No Hit
TTGGCG36450.10056681609598793No Hit
ACAGGT36270.10007018984366206No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)