FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5770292
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT3403095.897604488646328Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT74170.12853768925385403Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAATT564750.0130.198043
CTGCAAT528750.0129.873182
ACTGCAA536150.0128.544591
CGCAATT68850.0125.506153
CCGCAAT66950.0122.236182
ACCGCAA69100.0118.708511
GCAATTT728500.0113.283264
CAATTTC752900.0110.818615
AATTTCC772000.0110.123466
TTTCCAC772150.0109.522158
TTCCACA794650.0106.5569159
ATTTCCA798100.0106.324137
CGGCAAT14650.064.0545352
AGACCGG18850.050.9681131
ACGGCAA19450.049.0216261
ATTGCAA43000.048.9176251
GACCGGA19650.048.4960752
ATGCAAT61150.044.6100462
GGCAATT23050.042.9161223
TCCGATC24650.041.310445
Produced by FastQC (version 0.11.5)