FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15401401
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT203105813.187488592758543Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT922030.5986663161357852Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT718070.46623680533998174Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT614040.3986910022016828Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT479280.31119246878904067Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT191370.12425492979502319Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC171240.11118469027590412RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC170800.11089900198040425RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1166000.0126.290792
ACTGCAA1206650.0122.356491
TGCAATT1314200.0121.6945343
CGCAATT151950.0113.6135943
CCGCAAT146000.0112.4854352
GCAATTT1650300.0108.561244
AATTTCC1817600.0107.408526
CAATTTC1755950.0106.354975
GACCGGA116800.0103.87282
TTTCCAC1875950.0103.406368
ATTTCCA1886400.0103.275347
ACCGCAA159650.0102.9002761
AGACCGG123600.098.567241
TTCCACA1978450.098.20299
ACCGGAA135700.089.298813
AGATTGG199600.082.341171
GATTGGA201850.081.038622
ACGGAAG128650.080.277584
AGAACGG131600.079.3186951
GAACGGA139050.075.2115552
Produced by FastQC (version 0.11.5)