FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005479651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5685190
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT317839155.90650444400275Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT844321.4851218692778956Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT523140.9201803281860413Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT307050.5400874904796498Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT231230.4067234340453001Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC181800.3197782308067101RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC179660.3160140646134958RNA PCR Primer, Index 16 (96% over 25bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT177170.3116342637625128Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC170100.2991984436755852Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC135880.23900696370745744Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC95900.1686838962286221RNA PCR Primer, Index 16 (100% over 22bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC90310.15885133126597353TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGCAACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC84570.14875492287856693TruSeq Adapter, Index 16 (95% over 22bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT79400.13966111950524082Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGT79110.13915102221737533Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATNGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT77670.13661812533969842Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACAGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC74020.13019793533725346TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTAGTATGCCGTCTT70360.12376015577315798Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC64670.11375169519400408Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCG61750.10861554319204811Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)