FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13407411
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT270155720.14972913114993Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT792740.5912700073116278Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT562070.4192233683296499Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT464360.3463457635482346Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT406130.3029145597162644Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT303930.2266880608045804Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC223900.16699719282119418RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC220470.16443890621388424RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC168270.1255052149889341Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT134480.10030273555423938Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT909350.0121.681972
ACTGCAA911150.0121.608221
TGCAATT1069150.0116.344393
CGCAATT126800.0108.115933
CCGCAAT122550.0104.2933042
GCAATTT1378150.0101.220744
GACCGGA99200.099.9020162
AATTTCC1501150.099.875366
CAATTTC1466150.099.151785
AGACCGG102250.097.64161
TTTCCAC1527600.097.397368
ATTTCCA1549050.096.485697
TTCCACA1608150.092.59559
ACCGCAA141900.090.2266851
ACCGGAA117050.084.543193
AGATTGG165500.084.077981
GATTGGA168200.081.935772
AGAACGG109350.075.61971
ACGGAAG110350.074.434344
GAACGGA111400.073.863192
Produced by FastQC (version 0.11.5)