FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005479675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5652585
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2513364.446390456755625Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT105460.18656950757927568Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT96870.17137292053104905Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT77020.13625624382472798Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT69420.12281106785656473Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT333650.0123.929332
TGCAATT387150.0122.749833
ACTGCAA336600.0119.788721
GCAATTT477500.0115.505274
CAATTTC545200.0112.751175
AATTTCC579950.0111.5022056
CGCAATT46000.0111.353973
TTTCCAC602950.0106.4819648
ATTTCCA611500.0105.4435967
ACCGCAA41950.0103.0870061
TTCCACA631650.0101.896279
CCGCAAT43400.0101.8108752
CGGCAAT22550.086.8727952
GACCGGA24500.083.808282
AGACCGG26100.077.562781
ACGGCAA26850.070.26171
GGCAATT38550.063.990193
TGATCGG11200.062.841041
ACATCGG17950.062.250941
ACCGGAA34300.061.9772573
Produced by FastQC (version 0.11.5)