FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005479687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005479687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41221789
Sequences flagged as poor quality0
Sequence length25-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA5406801.3116364260658362No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC4584501.1121545452576063No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1303240.3161531878201599No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC897310.21767856800198557No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAG887240.21523568518581276No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG876110.21253565681004288No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC839240.2035913579587727No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCG807780.19595947182205026No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACG707630.1716640682431323No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT606790.14720127746032566No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG597630.1449791516811655No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA563880.13679173409965298No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG493050.11960907373525201No Hit
CAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGAGAAAACGCTAC486200.11794733120389317No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCA443640.10762269439591765No Hit
CGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG432680.1049639063457435No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)